The Shared Epitope Phenomenon – A potential impediment to virtual crossmatch accuracy.

With the implementation of the new Kidney Allocation System (KAS), there is increased reliance on a virtual crossmatch/histocompatibility risk assessment (vXM) for evaluating potential presence, as well as strength, of HLA antibodies against a potential donor.

The accuracy of such an assessment depends on the precision in the identification of the recipient’s antibody profile and the potential donor’s HLA typing. While the development of the single antigen bead (SAB) multiplex assay has improved the sensitivity and specificity of HLA-antibody detection, several limitations of the assay (specific to certain sensitized patients) can complicate accurate interpretation of results.

In this report, we focus on the “shared-epitope” phenomenon, a condition in which antibody strength can be underrepresented, or its presence completely missed, due to binding of the antibody to competing targets on multiple antigens (beads), effectively “diluting” the resulting MFI-readout. Here, we provide a relevant background to understand this phenomenon and present a couple of case studies illustrating how it can be investigated, leading to a more accurate histocompatibility consultation.

p38
MBS8533658-01mLAF405S 0.1mL(AF405S)
EUR 565

p38
MBS8533658-01mLAF610 0.1mL(AF610)
EUR 565

p38
MBS8533658-01mLAF635 0.1mL(AF635)
EUR 565

P38 mAb
MBS435304-005mL 0.05mL
EUR 415

P38 mAb
MBS435304-01mL 0.1mL
EUR 660

P38 mAb
MBS435304-5x01mL 5x0.1mL
EUR 2655

p38 MAPK
E8ET1702-65 100ul
EUR 275
Description: Available in various conjugation types.

Mxi2, p38
MBS603655-01mg 0.1mg
EUR 820

Mxi2, p38
MBS603655-5x01mg 5x0.1mg
EUR 3530

p38 MAPK
MBS8534372-01mL 0.1mL
EUR 345

p38 MAPK
MBS8534372-01mLAF405L 0.1mL(AF405L)
EUR 565

p38 MAPK
MBS8534372-01mLAF405S 0.1mL(AF405S)
EUR 565

p38 MAPK
MBS8534372-01mLAF610 0.1mL(AF610)
EUR 565

p38 MAPK
MBS8534372-01mLAF635 0.1mL(AF635)
EUR 565

p38 protein
E20-80078 20ug
EUR 275
Description: N/A

p38 protein
E20-80079 20ug
EUR 275
Description: N/A

p38 protein
MBS8526800-002mg 0.02mg
EUR 345

p38 protein
MBS8526800-5x002mg 5x0.02mg
EUR 1410

p38 protein
MBS8526801-002mg 0.02mg
EUR 345

p38 protein
MBS8526801-5x002mg 5x0.02mg
EUR 1410

p38 Antibody
35478 100ul
EUR 479

p38 Antibody
35478-100ul 100ul
EUR 468

p38 Antibody
48644 100ul
EUR 499

p38 Antibody
48644-100ul 100ul
EUR 399.6

p38 Antibody
48644-50ul 50ul
EUR 286.8

p38 Antibody
20-abx007541
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  • 30 ul
  • 100 ul
  • 200 ul

p38 Antibody
20-abx133223
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  • 30 ul
  • 100 ul
  • 200 ul

p38 Antibody
20-abx141038
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  • 30 ul
  • 100 ul
  • 200 ul

p38 Antibody
B7178-100ul 100μl
EUR 217
Description: p38 Rabbit Polyclonal Antibody

p38 Antibody
B7178-50ul 50μl
EUR 143.5
Description: p38 Rabbit Polyclonal Antibody

p38 Antibody
B0798-100ul 100μl
EUR 217
Description: p38 Rabbit Polyclonal Antibody

p38 Antibody
B0798-50ul 50μl
EUR 143.5
Description: p38 Rabbit Polyclonal Antibody

p38 Antibody
B0799-100ul 100μl
EUR 217
Description: p38 Rabbit Polyclonal Antibody

p38 Antibody
B0799-50ul 50μl
EUR 143.5
Description: p38 Rabbit Polyclonal Antibody

p38 Antibody
abx236076-100g 100 µg
EUR 350

p38 Antibody
abx236076-100ug 100 ug
EUR 577.2

p38 Antibody
E38PA4721 100ul
EUR 225
Description: Available in various conjugation types.

p38 Antibody
E38PA5060 100ul
EUR 225
Description: Available in various conjugation types.

p38 Antibody
E38PA5094 100ul
EUR 225
Description: Available in various conjugation types.

p38 Antibody
E38PA7126 100ul
EUR 225
Description: Available in various conjugation types.

p38 Antibody
E38PA3186 100ul
EUR 225
Description: Available in various conjugation types.

p38 Antibody
MBS5316121-01mL 0.1mL
EUR 460

p38 Antibody
MBS5316121-5x01mL 5x0.1mL
EUR 1920

p38 Antibody
MBS5316122-01mL 0.1mL
EUR 535

p38 Antibody
MBS5316122-5x01mL 5x0.1mL
EUR 2270

p38 Antibody
MBS805089-01mL 0.1mL
EUR 425

p38 Antibody
MBS805089-5x01mL 5x0.1mL
EUR 1350

p38 Antibody
MBS806203-0025mL 0.025mL
EUR 320

p38 Antibody
MBS8002013-0025mL 0.025mL
EUR 315

p38 Antibody
MBS8002013-5x0025mL 5x0.025mL
EUR 890

p38 Antibody
MBS8584173-01mL 0.1mL
EUR 305

p38 Antibody
MBS8584173-01mLAF405L 0.1mL(AF405L)
EUR 465

p38 Antibody
MBS8584173-01mLAF405S 0.1mL(AF405S)
EUR 465

p38 Antibody
MBS8584173-01mLAF610 0.1mL(AF610)
EUR 465

p38 Antibody
MBS8584173-01mLAF635 0.1mL(AF635)
EUR 465

p38 Antibody
MBS8582665-01mL 0.1mL
EUR 305

p38 Antibody
MBS8582665-01mLAF405L 0.1mL(AF405L)
EUR 465

p38 Antibody
MBS8582665-01mLAF405S 0.1mL(AF405S)
EUR 465

p38 Antibody
MBS8582665-01mLAF610 0.1mL(AF610)
EUR 465

p38 Antibody
MBS8582665-01mLAF635 0.1mL(AF635)
EUR 465

p38 Antibody
MBS8582905-01mL 0.1mL
EUR 305

p38 Antibody
MBS8582905-01mLAF405L 0.1mL(AF405L)
EUR 465

p38 Antibody
MBS8582905-01mLAF405S 0.1mL(AF405S)
EUR 465

p38 Antibody
MBS8582905-01mLAF610 0.1mL(AF610)
EUR 465

p38 Antibody
MBS8582905-01mLAF635 0.1mL(AF635)
EUR 465

p38 Antibody
MBS8582923-01mL 0.1mL
EUR 305

p38 Antibody
MBS8582923-01mLAF405L 0.1mL(AF405L)
EUR 465

p38 Antibody
MBS8582923-01mLAF405S 0.1mL(AF405S)
EUR 465

p38 Antibody
MBS8582923-01mLAF610 0.1mL(AF610)
EUR 465

p38 Antibody
MBS8582923-01mLAF635 0.1mL(AF635)
EUR 465

p38 Antibody
MBS8581787-01mL 0.1mL
EUR 305

p38 Antibody
MBS8581787-01mLAF405L 0.1mL(AF405L)
EUR 465

p38 Antibody
MBS8581787-01mLAF405S 0.1mL(AF405S)
EUR 465

p38 Antibody
MBS8581787-01mLAF610 0.1mL(AF610)
EUR 465

p38 Antibody
MBS8581787-01mLAF635 0.1mL(AF635)
EUR 465

p38 Antibody
MBS9508977-005mL 0.05mL
EUR 285

p38 Antibody
MBS9508977-01mL 0.1mL
EUR 385

p38 Antibody
MBS9508977-5x01mL 5x0.1mL
EUR 1590

p38 Antibody
MBS9435492-005mL 0.05mL
EUR 325

p38 Antibody
MBS9435492-01mL 0.1mL
EUR 435

p38 Antibody
MBS9435492-5x01mL 5x0.1mL
EUR 1810

p38 Antibody
MBS9409774-01mL 0.1mL
EUR 420

p38 Antibody
MBS9409774-5x01mL 5x0.1mL
EUR 1740

p38 Antibody
SPC-172D 0.1ml
EUR 161
Description: Rabbit Anti-Human p38 Polyclonal

p38 Antibody
SPC-172S 0.012ml Ask for price
Description: Rabbit Anti-Human p38 Polyclonal

p38 antibody
70R-51505 100 ul Ask for price

p38 antibody
70R-51506 100 ul Ask for price

p38 Substrate
MBS517838-1mg 1mg
EUR 205

p38 Substrate
MBS517838-5x1mg 5x1mg
EUR 675

MAP Kinase p38, Control Cell Extracts (Mitogen Activated Protein Kinase p38, MAPK p38)
MBS657552-10Blots 10Blots
EUR 430

MAP Kinase p38, Control Cell Extracts (Mitogen Activated Protein Kinase p38, MAPK p38)
MBS657552-5x10Blots 5x10Blots
EUR 1785

MAP Kinase p38, phosphorylated (Thr180, Ty182) (Mitogen Activated Protein Kinase p38, MAPK p38)
MBS633592-01mL 0.1mL
EUR 695

MAP Kinase p38, phosphorylated (Thr180, Ty182) (Mitogen Activated Protein Kinase p38, MAPK p38)
MBS633592-5x01mL 5x0.1mL
EUR 2985

MAP Kinase p38, phosphorylated (Thr180, Tyr182) (Mitogen Activated Protein Kinase p38, MAPK p38)
MBS630803-02mL 0.2mL
EUR 650

MAP Kinase p38, phosphorylated (Thr180, Tyr182) (Mitogen Activated Protein Kinase p38, MAPK p38)
MBS630803-06mL 0.6mL
EUR 1175

MAP Kinase p38, phosphorylated (Thr180, Tyr182) (Mitogen Activated Protein Kinase p38, MAPK p38)
MBS630803-5x06mL 5x0.6mL
EUR 5140

MAP Kinase p38, phosphorylated (Thr180, Ty182) (Mitogen Activated Protein Kinase p38, MAPK p38)
MBS632290-02mL 0.2mL
EUR 660

MAP Kinase p38, phosphorylated (Thr180, Ty182) (Mitogen Activated Protein Kinase p38, MAPK p38)
MBS632290-5x02mL 5x0.2mL
EUR 2810

MAP Kinase p38, phosphorylated (Thr180, Tyr182) (Mitogen Activated Protein Kinase p38, MAPK p38)
MBS627818-10Blots 10Blots
EUR 875

MAP Kinase p38, phosphorylated (Thr180, Tyr182) (Mitogen Activated Protein Kinase p38, MAPK p38)
MBS627818-5x10Blots 5x10Blots
EUR 3785

SB-203580 p38 MAPK(p38 MAP kinase) Inhibitor
E1B1863 1mg
EUR 85

SB-203580 p38 MAPK (p38 MAP kinase) Inhibitor
MBS8579825-1mg 1mg
EUR 180

SB-203580 p38 MAPK (p38 MAP kinase) Inhibitor
MBS8579825-5x1mg 5x1mg
EUR 660

p38 (411-425) M.leprae
MBS8246858-10mg 10mg
EUR 675

p38 (411-425) M.leprae
MBS8246858-1mg 1mg
EUR 255

p38 (411-425) M.leprae
MBS8246858-5mg 5mg
EUR 480

p38 (411-425) M.leprae
MBS8246858-5x10mg 5x10mg
EUR 2955

MAP Kinase p38, phosphorylated (Thr180, Ty182) (Mitogen Activated Protein Kinase p38, MAPK p38) (Agarose)
MBS631660-04mL 0.4mL
EUR 670

MAP Kinase p38, phosphorylated (Thr180, Ty182) (Mitogen Activated Protein Kinase p38, MAPK p38) (Agarose)
MBS631660-5x04mL 5x0.4mL
EUR 2870

Anti - p38-a
MBS684252-002mL 0.02mL
EUR 270

Anti - p38-a
MBS684252-005mL 0.05mL
EUR 485

Anti - p38-a
MBS684252-01mL 0.1mL
EUR 720

Anti - p38-a
MBS684252-5x01mL 5x0.1mL
EUR 2925

Anti - p38-b
MBS684253-002mL 0.02mL
EUR 270

Anti - p38-b
MBS684253-005mL 0.05mL
EUR 485

Anti - p38-b
MBS684253-01mL 0.1mL
EUR 720

Anti - p38-b
MBS684253-5x01mL 5x0.1mL
EUR 2925

Anti - p38-g
MBS684254-002mL 0.02mL
EUR 270

Anti - p38-g
MBS684254-005mL 0.05mL
EUR 485

Anti - p38-g
MBS684254-01mL 0.1mL
EUR 720

Anti - p38-g
MBS684254-5x01mL 5x0.1mL
EUR 2925

Anti - p38-d
MBS684255-002mL 0.02mL
EUR 270

Anti - p38-d
MBS684255-005mL 0.05mL
EUR 485

Anti - p38-d
MBS684255-01mL 0.1mL
EUR 720

Anti - p38-d
MBS684255-5x01mL 5x0.1mL
EUR 2925

p38 Mouse mAb
E2222580 100ul
EUR 255
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

p38 Mouse mAb
E2220879 100ul
EUR 225
Description: Available in various conjugation types.

p38 Mouse mAb
MBS8539086-01mL 0.1mL
EUR 305

p38 Mouse mAb
MBS8539086-01mLAF405L 0.1mL(AF405L)
EUR 565

p38 Mouse mAb
MBS8539086-01mLAF405S 0.1mL(AF405S)
EUR 565

p38 Mouse mAb
MBS8539086-01mLAF610 0.1mL(AF610)
EUR 565

p38 Mouse mAb
MBS8539086-01mLAF635 0.1mL(AF635)
EUR 565

p38 Rabbit pAb
E2340697 100ul
EUR 225
Description: Available in various conjugation types.

p38 Rabbit pAb
E2380881 100ul
EUR 225
Description: Available in various conjugation types.

p38 Rabbit pAb
E2381051 100ul
EUR 225
Description: Available in various conjugation types.

p38 Rabbit mAb
E38A4061 100ug/100ul
EUR 255

p38 Rabbit mAb
E2R25239 100μl
EUR 225
Description: Available in various conjugation types.

p38 Rabbit pAb
E2251338 100ul
EUR 225
Description: Available in various conjugation types.

p38 Rabbit pAb
E2251719 100ul
EUR 225
Description: Available in various conjugation types.

p38 Rabbit mAb
E2R27123 100μl
EUR 225
Description: Available in various conjugation types.