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Gynecologic cytology, Lung, Thyroid, Liver & Pancreas

Gynecologic cytology, Lung, Thyroid, Liver & Pancreas

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  • Gynecologic cytology on conventional and liquid-based preparations: a comprehensive review of similarities and differences.
  • Monitoring and ordering practices for human papillomavirus in cervical cytology: findings from the College of American Pathologists Gynecologic Cytopathology Quality Consensus Conference working group 5.
  • 5 Tips For Immunofluorescence (If)
  • Systematic Elucidation of the Potential Mechanisms of Core Chinese Materia Medicas in Treating Liver Cancer Based on Network Pharmacology.
  • Systematic Elucidation of the Potential Mechanisms of Core Chinese Materia Medicas in Treating Liver Cancer Based on Network Pharmacology.
  • TNF-alpha inhibition ameliorates HDV-induced liver damage in a mouse model of acute severe infection.
  • Hepatoprotective and antioxidant activity of hydroalcoholic extract of Stachys pilifera. Benth on acetaminophen-induced liver toxicity in male rats.

dna hr block

Comparing the performance of FAM19A4 methylation analysis, cytology and HPV16/18 genotyping for the detection of cervical (pre)cancer in high-risk HPV-positive women of a gynecologic outpatient population (COMETH study).

Posted by Rosa Wheeler on April 23, 2021 in Active proteins, Antibodies and other Reagents, antibody, assay-kits, biochemical, blocking-peptide, cell-line, culture-media, Datasheets Msds, elisa-kits, enzyme, Hepatoprotective and antioxidant activity of hydroalcoholic extract of Stachys pilifera. Benth on acetaminophen-induced liver toxicity in male rats., Human Gene, human-elisa-kits, monoclonal-antibody, monoclonal-human-antibody, mouse-elisa-kits, peptide, polyclonal-antibody, polyclonal-human-mouse-rat-antibody, protein, rat-elisa-kits, reagents, recombinant-protein, restriction-enzyme, small-molecule, Systematic Elucidation of the Potential Mechanisms of Core Chinese Materia Medicas in Treating Liver Cancer Based on Network Pharmacology., TNF-alpha inhibition ameliorates HDV-induced liver damage in a mouse model of acute severe infection.
Comparing the performance of FAM19A4 methylation analysis, cytology and HPV16/18 genotyping for the detection of cervical (pre)cancer in high-risk HPV-positive women of a gynecologic outpatient population (COMETH study).

Recently, DNA methylation evaluation of FAM19A4 in cervical scrapes has been proven to adequately detect high-grade cervical intraepithelial neoplasia and cervical most cancers (≥ CIN3) in high-risk HPV (hrHPV)-positive women. Here, we in contrast the medical performance of FAM19A4 methylation evaluation to cytology and HPV16/18 genotyping, individually and in mixture, for ≥ CIN3 detection in hrHPV-positive women collaborating in a potential observational multi-center cohort research.

The research population comprised hrHPV-positive women aged 18-66 years, visiting a gynecological outpatient clinic. From these women, cervical scrapes and colposcopy-directed biopsies (for histological affirmation) had been obtained. Cervical scrapes had been analyzed for FAM19A4 gene promoter methylation, cytology and HPV16/18 genotyping. Both sensitivity and specificity of FAM19A4 methylation evaluation had been related to age.

Methylation evaluation was carried out by quantitative methylation-specific PCR (qMSP). Sensitivities and specificities for ≥ CIN3 had been in contrast between exams. Stratified analyses had been carried out for variables that probably affect marker performance. Of all 508 hrHPV-positive women, the sensitivities for ≥ CIN3 of cytology, FAM19A4 methylation evaluation, and cytology mixed with HPV16/18 genotyping had been 85.6, 75.6 and 92.2%, respectively, with corresponding specificities of 49.8, 71.1 and 29.4%, respectively. 

In women ≥ 30 years (n = 287), ≥ CIN3 sensitivity of FAM19A4 methylation evaluation was 88.3% which was noninferior to that of cytology at a considerably larger specificity [62.1% (95%CI: 55.8-68.4) compared to 47.6%. In conclusion, among hrHPV-positive women from an outpatient population aged ≥ 30 years, methylation analysis of FAM19A4 is an attractive marker for the identification of women with ≥ CIN3.

Cytology has been the mainstay of cervical dysplasia and cancer screening in the United States. The specificity of a woman harboring a high-grade lesion when identified as high-grade squamous intraepithelial lesion on Pap test is high; however, the test suffers from low sensitivity. Epidemiology studies have demonstrated that human papillomavirus (HPV) types 16 and 18 account for most cervical squamous cell carcinomas. Tests have been developed to identify high-risk HPV, some specifically to identify HPV 16 and 18.

Young investigator challenge: Comparison of 2 different methods of manual slide screening in semiautomated gynecologic cytology.

Negative gynecologic cytology cases (ie, those diagnosed as negative for intraepithelial lesion or malignancy) are manually reviewed by 2 methods using semiautomated screening: 1) immediate full slide review (FSR) after fields-of-view analysis (FOV) (FOV + FSR), and 2) quality-assurance/high-risk, quintile-directed full manual review (FMR). Data supporting current guidelines were limited.
The authors investigated FMR, FOV + FSR, and the review process in general. Gynecologic cytology cases from 2009 to 2014 at Massachusetts General Hospital were analyzed. The data comprised 93,169 patients, 194,656 specimens, and 49,979 human papillomavirus (HPV) tests. Simultaneous to the increase in HPV detection methods, interdisciplinary groups are making recommendations on the managerial use of the tests.
In patients who underwent FMR, the epithelial cell abnormality (ECA) rate was correlated with the HPV-positive rate (correlation coefficient [r(2) ] = 0.82; Y = 0.19X + 0.02), and each charges decreased with age. For sufferers who underwent FOV + FSR, the ECA charge was additionally associated to the HPV-positive charge, and each charges decreased with age. The slope of the line evaluating the FMR ECA charge with the FMR HPV-positive charge could be a helpful surrogate marker of screening accuracy.
The FMR group had comparable HPV-positive charges in comparison with the FOV + FSR group (2%-52% vs 9%-68%, respectively). HPV-positive sufferers had a larger threat of ECA than HPV-negative sufferers (40% vs 8%; P < .0001). Currently, guide evaluation allocates sources inefficiently, to older, HPV-negative sufferers as in comparison with youthful, HPV-positive sufferers. FMR ought to be assigned based on HPV standing and age.
Additionally, too many sufferers who’ve HPV-positive specimens with ECA proceed from major screening to FMR. This imbalance might be noticed in the slope of the line evaluating the FMR ECA charge with the HPV-positive charge (which could function a surrogate marker for adequacy of FOV + FSR screening). Therefore, laboratories ought to attempt to scale back the quantity of HPV-positive circumstances that attain FMR. Current evaluation procedures commit too few sources to youthful sufferers.
Comparing the performance of FAM19A4 methylation analysis, cytology and HPV16/18 genotyping for the detection of cervical (pre)cancer in high-risk HPV-positive women of a gynecologic outpatient population (COMETH study).

Cytology for the detection of early recurrence of gynecologic most cancers in the vaginal vault.

The actual profit of follow-up cervical cytology in women handled for gynecological most cancers is unclear. This research was designed to evaluate the charge of success of cytological examinations in the detection of early vaginal recurrence of gynecological most cancers in women discovered by different strategies to have vaginal recurrence of cervical and endometrial most cancers. Records of cytological examinations.
Records from 1979 to 2010. Sixteen women (48.5%) had symptomatic vaginal recurrence related to distant metastases, whereas 17 (51.5%) had vaginal recurrence solely. Cytology was destructive in 12 women (36.4%) with each symptomatic and asymptomatic recurrence and optimistic in the different 21 (63.6%). In 9 of these 21 women (42.9%), the illness was restricted to the vaginal vault, whereas the remaining 12 (57.1%) introduced with vaginal lesions related to distant metastases. Cytology was optimistic in 9 of the 17 (52.9%) women whose recurrence was restricted to the vaginal vault and destructive in 8 (47.1%).

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Vaginal cytology yielded false-negative outcomes in nearly half of the women with vaginal recurrence of gynecological most cancers. Patents of strategies used for early prognosis and detection of immortalization of cervical most cancers are additionally reviewed in this text.

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Recent Announcements

  • Significance of Malignant Peritoneal Cytology on the Survival of Women with Early-Stage Cervical Cancer: A Japanese Gynecologic Oncology Group Study. May 22, 2021
  • Cervical cancer screening by molecular Pap-transformation of gynecologic cytology. May 8, 2021
  • Comparing the performance of FAM19A4 methylation analysis, cytology and HPV16/18 genotyping for the detection of cervical (pre)cancer in high-risk HPV-positive women of a gynecologic outpatient population (COMETH study). April 23, 2021
  • Prognostic value of peritoneal washing cytology in gynecologic malignancies: a controversial issue. April 16, 2021
  • Gynecologic cytology on conventional and liquid-based preparations: a comprehensive review of similarities and differences. March 6, 2021
  • Monitoring and ordering practices for human papillomavirus in cervical cytology: findings from the College of American Pathologists Gynecologic Cytopathology Quality Consensus Conference working group 5. March 6, 2021

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