ESAT 6 like protein EsxO

Improved outcomes for index cholecystectomy for acute cholecystitis following a dedicated emergency surgery and trauma service (ESAT).

Journal: European journal of trauma and emergency surgery : official publication of the European Trauma Society

Februar/5/2020
Early laparoscopic cholecystectomy (ELC) has shown to reduce length of stay and improve patients’ satisfaction as compared to delayed laparoscopic cholecystectomy (DLC). However, logistics and manpower limitations often preclude ELC.A retrospective study was conducted in a single institute to compare outcomes of AC before (August 2013-2014) and after (August 2017-2018) establishment of emergency surgery and trauma (ESAT).There were 82 patients in pre-ESAT period and 172 patients in ESAT period. Mean age was 52.3 ± 11.6 and 55.7 ± 13.8 years, respectively, p = 0.369. There were more patients with moderate-severe grading of cholecystitis based on Tokyo Guidelines (TG 18) in ESAT 143/172 (83.1%) as compared to pre-ESAT 65/82 (79.3%), p = 0.042. Index cholecystectomy was performed in 145/172 (84.3%) of patients in the ESAT vs 34/82 (41.5%) of patients in the pre-ESAT period (p = 0.001). Time interval between booking to surgery was 180 ± 56 min in ESAT vs 197 ± 98 min in pre-ESAT, p = 0.014. Operative duration was shorter in ESAT 121 ± 38.5 min vs 139 ± 53.4, in pre-ESAT period, p = 0.030. Conversion rates were lower in ESAT (4/172, 2.3%) vs (9/72, 11%) in pre-ESAT, p = 0.003. Length of stay was shorter in ESAT (DLC 1.89 ± 1.6 and ELC ± 2.9 days) as compared to pre-ESAT (DLC 4.55 ± 2.2 and ELC 5.03 ± 2.6 days), p = 0.001. 30-day readmissions were lower in ESAT (3/172, 1.7%) vs pre-ESAT (8/72, 9.8%).The ESAT model provided more early laparoscopic cholecystectomies with improved efficiency and clinical outcomes.
Si Goh; Clement Chia; Jing Ong; John Quek; Woan Lim; Kok Tan; Jerry Goo

EspM Is a Conserved Transcription Factor That Regulates Gene Expression in Response to the ESX-1 System.

Journal: mBio

Februar/5/2020
Pathogenic mycobacteria encounter multiple environments during macrophage infection. Temporally, the bacteria are engulfed into the phagosome, lyse the phagosomal membrane, and interact with the cytosol before spreading to another cell. Virulence factors secreted by the mycobacterial ESX-1 (ESAT-6-system-1) secretion system mediate the essential transition from the phagosome to the cytosol. It was recently discovered that the ESX-1 system also regulates mycobacterial gene expression in Mycobacterium marinum (R. E. Bosserman, T. T. Nguyen, K. G. Sanchez, A. E. Chirakos, et al., Proc Natl Acad Sci U S A 114:E10772-E10781, 2017, https://doi.org/10.1073/pnas.1710167114), a nontuberculous mycobacterial pathogen, and in the human-pathogenic species M. tuberculosis (A. M. Abdallah, E. M. Weerdenburg, Q. Guan, R. Ummels, et al., PLoS One 14:e0211003, 2019, https://doi.org/10.1371/journal.pone.0211003). It is not known how the ESX-1 system regulates gene expression. Here, we identify the first transcription factor required for the ESX-1-dependent transcriptional response in pathogenic mycobacteria. We demonstrate that the gene divergently transcribed from the whiB6 gene and adjacent to the ESX-1 locus in mycobacterial pathogens encodes a conserved transcription factor (MMAR_5438, Rv3863, now espM). We prove that EspM from both M. marinum and M. tuberculosis directly and specifically binds the whiB6-espM intergenic region. We show that EspM is required for ESX-1-dependent repression of whiB6 expression and for the regulation of ESX-1-associated gene expression. Finally, we demonstrate that EspM functions to fine-tune ESX-1 activity in M. marinum Taking the data together, this report extends the esx-1 locus, defines a conserved regulator of the ESX-1 virulence pathway, and begins to elucidate how the ESX-1 system regulates gene expression.IMPORTANCE Mycobacterial pathogens use the ESX-1 system to transport protein substrates that mediate essential interactions with the host during infection. We previously demonstrated that in addition to transporting proteins, the ESX-1 secretion system regulates gene expression. Here, we identify a conserved transcription factor that regulates gene expression in response to the ESX-1 system. We demonstrate that this transcription factor is functionally conserved in M. marinum, a pathogen of ectothermic animals; M. tuberculosis, the human-pathogenic species that causes tuberculosis; and M. smegmatis, a nonpathogenic mycobacterial species. These findings provide the first mechanistic insight into how the ESX-1 system elicits a transcriptional response, a function of this protein transport system that was previously unknown.
Kevin Sanchez; Micah Ferrell; Alexandra Chirakos; Kathleen Nicholson; Robert Abramovitch; Matthew Champion; Patricia Champion

TB ESAT from EnoGene
E61H01301 | 100ug: 431.25 EUR
PepPool: TB (ESAT-6), scanning from Mabtech AB
3624-1 | One vial with 25 ug of each peptide: 409.50 EUR
M. Tuberculosis ESAT-6 Antigen from Creative Diagnostics
DAGA-187 | 100 µg: 868.80 EUR
TB-16&TB-38 & ESAT from EnoGene
E61H01303 | 100ug: 255.00 EUR
ESAT Recombinant Protein (Human) from ABM
RP057123 | 100 ug: Ask for price
ESAT ORF Vector (Human) (pORF) from ABM
ORF019042 | 1.0 ug DNA: Ask for price
Recombinant Mycobacterium tuberculosis ESAT-6 from Creative Diagnostics
DAGH171 | 1 mg: 630.00 EUR
Description: Recombinant
ESAT-6-like protein EsxB Antibody from Abbexa
20-abx110662 | [ 100 ug: Ask for price ] [ 1 mg: Ask for price ] [ 200 ug: Ask for price ] [ 20 ug: Ask for price ] [ 50 ug: Ask for price ]
ESAT-6-like protein EsxB Antibody (HRP) from Abbexa
20-abx109228 | [ 100 ug: Ask for price ] [ 1 mg: Ask for price ] [ 200 ug: Ask for price ] [ 20 ug: Ask for price ] [ 50 ug: Ask for price ]
ESAT-6-like protein EsxB Antibody (FITC) from Abbexa
20-abx107810 | [ 100 ug: Ask for price ] [ 1 mg: Ask for price ] [ 200 ug: Ask for price ] [ 20 ug: Ask for price ] [ 50 ug: Ask for price ]
ESAT-6-Like Protein EsxB (esxB) Antibody from Abbexa
abx110662-100l | 100 µl: 162.50 EUR
ESAT-6-Like Protein EsxH (esxH) Antibody from Abbexa
abx140580-100g | 100 µg: 350.00 EUR
ESAT-6-like protein EsxB Antibody (Biotin) from Abbexa
20-abx106398 | [ 100 ug: Ask for price ] [ 1 mg: Ask for price ] [ 200 ug: Ask for price ] [ 20 ug: Ask for price ] [ 50 ug: Ask for price ]
ESAT 3'UTR GFP Stable Cell Line from ABM
TU057053 | 1.0 ml: 2799.60 EUR
ESAT sgRNA CRISPR Lentivector set (Human) from ABM
K0696001 | 3 x 1.0 ug: 406.80 EUR
ESAT-6-Like Protein EsxB (esxB) Antibody (HRP) from Abbexa
abx109228-100l | 100 µl: 162.50 EUR
ESAT-6-Like Protein EsxB (esxB) Antibody (FITC) from Abbexa
abx107810-100g | 100 µg: 362.50 EUR
ESAT-6-Like Protein EsxB (esxB) Antibody (FITC) from Abbexa
abx107810-20g | 20 µg: 162.50 EUR
ESAT-6-Like Protein EsxB (esxB) Antibody (FITC) from Abbexa
abx107810-50g | 50 µg: 250.00 EUR
ESAT-6-Like Protein EsxB (esxB) Antibody (Biotin) from Abbexa
abx106398-100g | 100 µg: 362.50 EUR
ESAT-6-Like Protein EsxB (esxB) Antibody (Biotin) from Abbexa
abx106398-20g | 20 µg: 162.50 EUR
ESAT-6-Like Protein EsxB (esxB) Antibody (Biotin) from Abbexa
abx106398-50g | 50 µg: 250.00 EUR
Mycobacterium tuberculosis antigen EsaT-6 (Rv3875) Antibody from Abbexa
abx140048-01mg | 0.1 mg: 510.00 EUR
Mycobacterium tuberculosis antigen EsaT-6 (Rv3875) Antibody from Abbexa
abx140048-100g | 100 µg: 350.00 EUR
ESAT Protein Vector (Human) (pPM-C-HA) from ABM
PV076167 | 500 ng: Ask for price
ESAT Protein Vector (Human) (pPB-C-His) from ABM
PV076165 | 500 ng: Ask for price
ESAT Protein Vector (Human) (pPB-N-His) from ABM
PV076166 | 500 ng: Ask for price
ESAT Protein Vector (Human) (pPM-C-His) from ABM
PV076168 | 500 ng: Ask for price
ESAT 3'UTR Luciferase Stable Cell Line from ABM
TU007053 | 1.0 ml: 2799.60 EUR
ESAT sgRNA CRISPR Lentivector (Human) (Target 1) from ABM
K0696002 | 1.0 ug DNA: 184.80 EUR
ESAT sgRNA CRISPR Lentivector (Human) (Target 2) from ABM
K0696003 | 1.0 ug DNA: 184.80 EUR
ESAT sgRNA CRISPR Lentivector (Human) (Target 3) from ABM
K0696004 | 1.0 ug DNA: 184.80 EUR
Mycobacterium tuberculosis ESAT-6-like protein esxB (esxB) from Cusabio
1-CSB-YP363658MVZ | [ 100ug: Ask for price ] [ 10ug: Ask for price ] [ 1MG: Ask for price ] [ 200ug: Ask for price ] [ 500ug: Ask for price ] [ 50ug: Ask for price ]
Description: Recombinant Mycobacterium tuberculosis ESAT-6-like protein esxB(esxB) expressed in Yeast
Mycobacterium tuberculosis ESAT-6-like protein EsxB (esxB) from Cusabio
1-CSB-EP363658MVZ | [ 100ug: Ask for price ] [ 10ug: Ask for price ] [ 1MG: Ask for price ] [ 200ug: Ask for price ] [ 500ug: Ask for price ] [ 50ug: Ask for price ]
Description: Recombinant Mycobacterium tuberculosis ESAT-6-like protein EsxB(esxB) expressed in E.coli
Mycobacterium tuberculosis ESAT-6-like protein EsxH (esxH) from Cusabio
1-CSB-EP363659MVZ | [ 100ug: Ask for price ] [ 10ug: Ask for price ] [ 1MG: Ask for price ] [ 200ug: Ask for price ] [ 500ug: Ask for price ] [ 50ug: Ask for price ]
Description: Recombinant Mycobacterium tuberculosis ESAT-6-like protein EsxH(esxH),partial expressed in E.coli
Recombinant Mycobacteroides abscessus ESAT-6-like protein (esxT) from Cusabio
CSB-EP4812GTR | 11120 mg: Ask for price
Recombinant Mycobacteroides abscessus ESAT-6-like protein (D2E76_02620) from Cusabio
CSB-EP4811GTR | 11119 mg: Ask for price
ESAT-6 Secretion System Extracellular Protein A (ESXA) Antibody from Abbexa
20-abx300131 | [ 100 ug: Ask for price ] [ 1 mg: Ask for price ] [ 200 ug: Ask for price ] [ 20 ug: Ask for price ] [ 50 ug: Ask for price ]
ESAT-6 Secretion System Extracellular Protein A (esxA) Antibody from Abbexa
abx300131-100g | 100 µg: 362.50 EUR
ESAT-6 Secretion System Extracellular Protein A (esxA) Antibody from Abbexa
abx300131-20g | 20 µg: 162.50 EUR
ESAT-6 Secretion System Extracellular Protein A (esxA) Antibody from Abbexa
abx300131-50g | 50 µg: 250.00 EUR
ESAT-6, with the modified C-terminus (His6-Tag) Protein from Abbexa
20-abx169018 | [ 10 ug: Ask for price ] [ 120 µg: Ask for price ] [ 1 mg: Ask for price ]
ESAT Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) from ABM
LV724667 | 1.0 ug DNA: Ask for price
ESAT Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) from ABM
LV724671 | 1.0 ug DNA: Ask for price
ELISpot Path: TB (ESAT-6+CFP-10+EspC) Human IFN-gamma from Mabtech AB
3420-2AST-P4-1 | 1 plate: 1380.75 EUR
Monoclonal Anti-Mycobacterium tuberculosis antigen (6kDa/ESAT-6) IgG from Alpha Diagnostics
MTB061-M | 100 ul: 578.40 EUR
ESAT Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) from ABM
LV724672 | 1.0 ug DNA: Ask for price
Recombinant Mycobacterium tuberculosis ESAT-6-like protein esxB (esxB) from Cusabio
CSB-YP363658MVZ | 6400 mg: Ask for price
Recombinant Mycobacterium tuberculosis ESAT-6-like protein esxB(esxB) from SAB
AP72990 | each: Ask for price
Recombinant Mycobacterium tuberculosis ESAT-6-like protein EsxB(esxB) from SAB
AP76578 | 1mg: 2826.00 EUR
Recombinant Mycobacterium tuberculosis ESAT-6-like protein EsxB (esxB) from Cusabio
CSB-EP363658MVZ | 3423 mg: Ask for price
Recombinant Mycobacterium tuberculosis ESAT-6-like protein EsxB (esxB) from Cusabio
CSB-EP363658MVZb2 | 3424 mg: Ask for price
Recombinant Mycobacterium tuberculosis ESAT-6-like protein esxB (esxB) from Biomatik Corporation
RPC25281-100ug | 100ug: 817.30 EUR
Recombinant Mycobacterium tuberculosis ESAT-6-like protein esxB (esxB) from Biomatik Corporation
RPC25281-20ug | 20ug: 479.60 EUR
Recombinant Mycobacterium tuberculosis ESAT-6-like protein EsxB (esxB) from Biomatik Corporation
RPC22792-100ug | 100ug: 721.60 EUR
Recombinant Mycobacterium tuberculosis ESAT-6-like protein EsxB (esxB) from Biomatik Corporation
RPC22792-1mg | 1mg: 2884.70 EUR
Recombinant Mycobacterium tuberculosis ESAT-6-like protein EsxB (esxB) from Biomatik Corporation
RPC22792-20ug | 20ug: 448.10 EUR
Recombinant Mycobacterium tuberculosis ESAT-6-like protein EsxB (esxB) from Biomatik Corporation
RPC28769-100ug | 100ug: 765.50 EUR
Recombinant Mycobacterium tuberculosis ESAT-6-like protein EsxB (esxB) from Biomatik Corporation
RPC28769-1mg | 1mg: 2959.20 EUR
Recombinant Mycobacterium tuberculosis ESAT-6-like protein EsxB (esxB) from Biomatik Corporation
RPC28769-20ug | 20ug: 465.60 EUR
ESAT-6 Secretion System Extracellular Protein A (ESXA) Antibody (HRP) from Abbexa
20-abx300380 | [ 100 ug: Ask for price ] [ 1 mg: Ask for price ] [ 200 ug: Ask for price ] [ 20 ug: Ask for price ] [ 50 ug: Ask for price ]
Recombinant Mycobacterium Tuberculosis ESAT-6 Protein (aa 1-99) [His] from Creative Biolabs
VAng-Lsx06137-100g | 100 µg: 717.60 EUR
Description: Mycobacterium Tuberculosis (H37Rv) ESAT-6, recombinant protein from E. coli.
Recombinant Mycobacterium Tuberculosis ESAT-6 Protein (aa 1-99) [His] from Creative Biolabs
VAng-Lsx06137-1mg | 1 mg: 3686.40 EUR
Description: Mycobacterium Tuberculosis (H37Rv) ESAT-6, recombinant protein from E. coli.
ESAT-6 Secretion System Extracellular Protein A (esxA) Antibody (HRP) from Abbexa
abx300380-100g | 100 µg: 362.50 EUR
ESAT-6 Secretion System Extracellular Protein A (esxA) Antibody (HRP) from Abbexa
abx300380-20g | 20 µg: 162.50 EUR
ESAT-6 Secretion System Extracellular Protein A (esxA) Antibody (HRP) from Abbexa
abx300380-50g | 50 µg: 250.00 EUR
ESAT-6 Secretion System Extracellular Protein A (ESXA) Antibody (FITC) from Abbexa
20-abx300381 | [ 100 ug: Ask for price ] [ 1 mg: Ask for price ] [ 200 ug: Ask for price ] [ 20 ug: Ask for price ] [ 50 ug: Ask for price ]
ESAT-6 Secretion System Extracellular Protein A (esxA) Antibody (FITC) from Abbexa
abx300381-100g | 100 µg: 362.50 EUR
ESAT-6 Secretion System Extracellular Protein A (esxA) Antibody (FITC) from Abbexa
abx300381-20g | 20 µg: 162.50 EUR
ESAT-6 Secretion System Extracellular Protein A (esxA) Antibody (FITC) from Abbexa
abx300381-50g | 50 µg: 250.00 EUR
ESAT-6 Secretion System Extracellular Protein A (ESXA) Antibody (Biotin) from Abbexa
20-abx300382 | [ 100 ug: Ask for price ] [ 1 mg: Ask for price ] [ 200 ug: Ask for price ] [ 20 ug: Ask for price ] [ 50 ug: Ask for price ]
ESAT-6 Secretion System Extracellular Protein A (esxA) Antibody (Biotin) from Abbexa
abx300382-100g | 100 µg: 362.50 EUR
ESAT-6 Secretion System Extracellular Protein A (esxA) Antibody (Biotin) from Abbexa
abx300382-20g | 20 µg: 162.50 EUR
ESAT-6 Secretion System Extracellular Protein A (esxA) Antibody (Biotin) from Abbexa
abx300382-50g | 50 µg: 250.00 EUR
Mycobacterium tuberculosis ESAT-6 like protein EsxG Control/blocking peptide from Alpha Diagnostics
AB-23071-P | 100ug: 196.80 EUR
Staphylococcus aureus ESAT-6 secretion system extracellular protein A (esxA) from Cusabio
1-CSB-EP763968SKW | [ 100ug: Ask for price ] [ 10ug: Ask for price ] [ 1MG: Ask for price ] [ 200ug: Ask for price ] [ 500ug: Ask for price ] [ 50ug: Ask for price ]
Description: Recombinant Staphylococcus aureus ESAT-6 secretion system extracellular protein A(esxA) expressed in E.coli
Recombinant purified (E. coli) Mycobacterium tuberculosis antigen (6kDa/ESAT-6) from Alpha Diagnostics
MTB06-R | 100 ug: 927.60 EUR
Recombinant Mycobacterium tuberculosis ESAT-6-like protein EsxH(esxH),partial from SAB
AP71172 | 1mg: 2826.00 EUR
Recombinant Mycobacterium tuberculosis ESAT-6-like protein EsxH (esxH), partial from Cusabio
CSB-EP363659MVZ | 3425 mg: Ask for price
Recombinant Mycobacterium tuberculosis ESAT-6-like protein EsxH (esxH) ,partial from Biomatik Corporation
RPC20136-100ug | 100ug: 721.60 EUR
Recombinant Mycobacterium tuberculosis ESAT-6-like protein EsxH (esxH) ,partial from Biomatik Corporation
RPC20136-1mg | 1mg: 2884.70 EUR
Recombinant Mycobacterium tuberculosis ESAT-6-like protein EsxH (esxH) ,partial from Biomatik Corporation
RPC20136-20ug | 20ug: 448.10 EUR
Mycobacterium tuberculosis (ESAT) mouse monoclonal antibody, clone RSB14 from Origene Technologies GmbH
3ES6-RSB14 | 1 mg: Ask for price
Mycobacterium tuberculosis (ESAT) mouse monoclonal antibody, clone RSB34 from Origene Technologies GmbH
3ES6-RSB34 | 1 mg: Ask for price
Recombinant Mycobacteroides abscessus subsp. abscessus ESAT-6-like protein (esxT) from Cusabio
CSB-EP4265GRN | 10053 mg: Ask for price
FluoroSpot Path: TB (ESAT-6+CFP-10+EspC) Human IFN-gamma/IL-2 from Mabtech AB
FSP-0102-P4-1 | 1 plate: 1569.75 EUR
ESAT sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human) from ABM
K0696005 | 3 x 1.0 ug: 451.20 EUR
Recombinant purified M. tuberculosis antigen (6kDa/ESAT-6) control for Western from Alpha Diagnostics
MTB061-C | 100 ul: 343.20 EUR