ESAT 6 like protein EsxO

Improved outcomes for index cholecystectomy for acute cholecystitis following a dedicated emergency surgery and trauma service (ESAT).

Journal: European journal of trauma and emergency surgery : official publication of the European Trauma Society

Februar/5/2020
Early laparoscopic cholecystectomy (ELC) has shown to reduce length of stay and improve patients’ satisfaction as compared to delayed laparoscopic cholecystectomy (DLC). However, logistics and manpower limitations often preclude ELC.A retrospective study was conducted in a single institute to compare outcomes of AC before (August 2013-2014) and after (August 2017-2018) establishment of emergency surgery and trauma (ESAT).There were 82 patients in pre-ESAT period and 172 patients in ESAT period. Mean age was 52.3 ± 11.6 and 55.7 ± 13.8 years, respectively, p = 0.369. There were more patients with moderate-severe grading of cholecystitis based on Tokyo Guidelines (TG 18) in ESAT 143/172 (83.1%) as compared to pre-ESAT 65/82 (79.3%), p = 0.042. Index cholecystectomy was performed in 145/172 (84.3%) of patients in the ESAT vs 34/82 (41.5%) of patients in the pre-ESAT period (p = 0.001). Time interval between booking to surgery was 180 ± 56 min in ESAT vs 197 ± 98 min in pre-ESAT, p = 0.014. Operative duration was shorter in ESAT 121 ± 38.5 min vs 139 ± 53.4, in pre-ESAT period, p = 0.030. Conversion rates were lower in ESAT (4/172, 2.3%) vs (9/72, 11%) in pre-ESAT, p = 0.003. Length of stay was shorter in ESAT (DLC 1.89 ± 1.6 and ELC ± 2.9 days) as compared to pre-ESAT (DLC 4.55 ± 2.2 and ELC 5.03 ± 2.6 days), p = 0.001. 30-day readmissions were lower in ESAT (3/172, 1.7%) vs pre-ESAT (8/72, 9.8%).The ESAT model provided more early laparoscopic cholecystectomies with improved efficiency and clinical outcomes.
Si Goh; Clement Chia; Jing Ong; John Quek; Woan Lim; Kok Tan; Jerry Goo

daganton
M Tuberculosis
Capilia Neo TB Tuberculosis detection stips. 10 stips per box or 100 strips per box


EspM Is a Conserved Transcription Factor That Regulates Gene Expression in Response to the ESX-1 System.

Journal: mBio

Februar/5/2020
Pathogenic mycobacteria encounter multiple environments during macrophage infection. Temporally, the bacteria are engulfed into the phagosome, lyse the phagosomal membrane, and interact with the cytosol before spreading to another cell. Virulence factors secreted by the mycobacterial ESX-1 (ESAT-6-system-1) secretion system mediate the essential transition from the phagosome to the cytosol. It was recently discovered that the ESX-1 system also regulates mycobacterial gene expression in Mycobacterium marinum (R. E. Bosserman, T. T. Nguyen, K. G. Sanchez, A. E. Chirakos, et al., Proc Natl Acad Sci U S A 114:E10772-E10781, 2017, https://doi.org/10.1073/pnas.1710167114), a nontuberculous mycobacterial pathogen, and in the human-pathogenic species M. tuberculosis (A. M. Abdallah, E. M. Weerdenburg, Q. Guan, R. Ummels, et al., PLoS One 14:e0211003, 2019, https://doi.org/10.1371/journal.pone.0211003). It is not known how the ESX-1 system regulates gene expression. Here, we identify the first transcription factor required for the ESX-1-dependent transcriptional response in pathogenic mycobacteria. We demonstrate that the gene divergently transcribed from the whiB6 gene and adjacent to the ESX-1 locus in mycobacterial pathogens encodes a conserved transcription factor (MMAR_5438, Rv3863, now espM). We prove that EspM from both M. marinum and M. tuberculosis directly and specifically binds the whiB6-espM intergenic region. We show that EspM is required for ESX-1-dependent repression of whiB6 expression and for the regulation of ESX-1-associated gene expression. Finally, we demonstrate that EspM functions to fine-tune ESX-1 activity in M. marinum Taking the data together, this report extends the esx-1 locus, defines a conserved regulator of the ESX-1 virulence pathway, and begins to elucidate how the ESX-1 system regulates gene expression.IMPORTANCE Mycobacterial pathogens use the ESX-1 system to transport protein substrates that mediate essential interactions with the host during infection. We previously demonstrated that in addition to transporting proteins, the ESX-1 secretion system regulates gene expression. Here, we identify a conserved transcription factor that regulates gene expression in response to the ESX-1 system. We demonstrate that this transcription factor is functionally conserved in M. marinum, a pathogen of ectothermic animals; M. tuberculosis, the human-pathogenic species that causes tuberculosis; and M. smegmatis, a nonpathogenic mycobacterial species. These findings provide the first mechanistic insight into how the ESX-1 system elicits a transcriptional response, a function of this protein transport system that was previously unknown.
Kevin Sanchez; Micah Ferrell; Alexandra Chirakos; Kathleen Nicholson; Robert Abramovitch; Matthew Champion; Patricia Champion

TB ESAT antigen
from EnoGene
E61H01301 | 100ug: 382.00 EUR
ESAT Recombinant Protein (Human)
from ABM
RP057123 | 100 ug: Ask for price
M. Tuberculosis ESAT-6 Antigen
from Creative Diagnostics
DAGA-187 | 100 µg: 724.00 EUR
TB-16&TB-38 & ESAT
from EnoGene
E61H01303 | 100ug: 343.00 EUR
ESAT ORF Vector (Human) (pORF)
from ABM
ORF019042 | 1.0 ug DNA: Ask for price
Anti-ESAT-6 (M. tuberculosis) Purified
from ExBio
11-491-C025 | 0.025 mg: 122.00 EUR
Anti-ESAT-6 (M. tuberculosis) Purified
from ExBio
11-491-C100 | 0.1 mg: 204.00 EUR
ESAT-6-like protein EsxB Antibody
from Abbexa
20-abx110662 | [ 100 ug: 411.00 EUR ] [ 1 mg: 1845.00 EUR ] [ 200 ug: 599.00 EUR ] [ 20 ug: 182.00 EUR ] [ 50 ug: 300.00 EUR ]
ESAT sgRNA CRISPR Lentivector set (Human)
from ABM
K0696001 | 3 x 1.0 ug: 339.00 EUR
ESAT-6-like protein EsxB Antibody (Biotin)
from Abbexa
20-abx106398 | [ 100 ug: 411.00 EUR ] [ 1 mg: 1845.00 EUR ] [ 200 ug: 599.00 EUR ] [ 20 ug: 182.00 EUR ] [ 50 ug: 300.00 EUR ]
ESAT-6-like protein EsxB Antibody (FITC)
from Abbexa
20-abx107810 | [ 100 ug: 411.00 EUR ] [ 1 mg: 1845.00 EUR ] [ 200 ug: 599.00 EUR ] [ 20 ug: 182.00 EUR ] [ 50 ug: 300.00 EUR ]
ESAT-6-like protein EsxB Antibody (HRP)
from Abbexa
20-abx109228 | [ 100 ug: 411.00 EUR ] [ 1 mg: 1845.00 EUR ] [ 200 ug: 599.00 EUR ] [ 20 ug: 182.00 EUR ] [ 50 ug: 300.00 EUR ]
Mycobacterium tuberculosis antigen EsaT-6 (Rv3875) Antibody
from Abbexa
abx140048-01mg | 0.1 mg: 425.00 EUR
ESAT sgRNA CRISPR Lentivector (Human) (Target 1)
from ABM
K0696002 | 1.0 ug DNA: 154.00 EUR
ESAT sgRNA CRISPR Lentivector (Human) (Target 2)
from ABM
K0696003 | 1.0 ug DNA: 154.00 EUR
ESAT sgRNA CRISPR Lentivector (Human) (Target 3)
from ABM
K0696004 | 1.0 ug DNA: 154.00 EUR
ESAT Protein Vector (Human) (pPB-C-His)
from ABM
PV076165 | 500 ng: Ask for price
ESAT Protein Vector (Human) (pPB-N-His)
from ABM
PV076166 | 500 ng: Ask for price
ESAT Protein Vector (Human) (pPM-C-HA)
from ABM
PV076167 | 500 ng: Ask for price
ESAT Protein Vector (Human) (pPM-C-His)
from ABM
PV076168 | 500 ng: Ask for price
ESAT 3'UTR GFP Stable Cell Line
from ABM
TU057053 | 1.0 ml: 2333.00 EUR
ESAT 3'UTR Luciferase Stable Cell Line
from ABM
TU007053 | 1.0 ml: 2333.00 EUR
Mycobacterium tuberculosis ESAT-6-like protein EsxB (esxB)
from Cusabio
1-CSB-EP363658MVZ | [ 100ug: 611.00 EUR ] [ 10ug: 309.00 EUR ] [ 1MG: 1827.00 EUR ] [ 200ug: 939.00 EUR ] [ 500ug: 1218.00 EUR ] [ 50ug: 397.00 EUR ]
Description: Recombinant Mycobacterium tuberculosis ESAT-6-like protein EsxB(esxB) expressed in E.coli
Mycobacterium tuberculosis ESAT-6-like protein EsxH (esxH)
from Cusabio
1-CSB-EP363659MVZ | [ 100ug: 611.00 EUR ] [ 10ug: 309.00 EUR ] [ 1MG: 1827.00 EUR ] [ 200ug: 939.00 EUR ] [ 500ug: 1218.00 EUR ] [ 50ug: 397.00 EUR ]
Description: Recombinant Mycobacterium tuberculosis ESAT-6-like protein EsxH(esxH),partial expressed in E.coli
Mycobacterium tuberculosis ESAT-6-like protein esxB (esxB)
from Cusabio
1-CSB-YP363658MVZ | [ 100ug: 679.00 EUR ] [ 10ug: 335.00 EUR ] [ 1MG: 2172.00 EUR ] [ 200ug: 1051.00 EUR ] [ 500ug: 1442.00 EUR ] [ 50ug: 435.00 EUR ]
Description: Recombinant Mycobacterium tuberculosis ESAT-6-like protein esxB(esxB) expressed in Yeast
ESAT Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV)
from ABM
LV724667 | 1.0 ug DNA: Ask for price
ESAT Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)
from ABM
LV724671 | 1.0 ug DNA: Ask for price
ESAT Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a)
from ABM
LV724672 | 1.0 ug DNA: Ask for price
ESAT-6 Secretion System Extracellular Protein A (ESXA) Antibody
from Abbexa
20-abx300131 | [ 100 ug: 411.00 EUR ] [ 1 mg: 1845.00 EUR ] [ 200 ug: 599.00 EUR ] [ 20 ug: 182.00 EUR ] [ 50 ug: 300.00 EUR ]
Monoclonal Anti-Mycobacterium tuberculosis antigen (6kDa/ESAT-6) IgG
from Alpha Diagnostics
MTB061-M | 100 ul: 482.00 EUR
Staphylococcus aureus ESAT-6 secretion system extracellular protein A (esxA)
from Cusabio
1-CSB-EP763968SKW | [ 100ug: 611.00 EUR ] [ 10ug: 309.00 EUR ] [ 1MG: 1827.00 EUR ] [ 200ug: 939.00 EUR ] [ 500ug: 1218.00 EUR ] [ 50ug: 397.00 EUR ]
Description: Recombinant Staphylococcus aureus ESAT-6 secretion system extracellular protein A(esxA) expressed in E.coli
ESAT-6, with the modified C-terminus (His6-Tag) Protein
from Abbexa
20-abx169018 | [ 10 ug: 328.00 EUR ] [ 120 µg: 913.00 EUR ] [ 1 mg: 4922.00 EUR ]
ESAT-6 Secretion System Extracellular Protein A (ESXA) Antibody (HRP)
from Abbexa
20-abx300380 | [ 100 ug: 411.00 EUR ] [ 1 mg: 1845.00 EUR ] [ 200 ug: 599.00 EUR ] [ 20 ug: 182.00 EUR ] [ 50 ug: 300.00 EUR ]
ESAT-6 Secretion System Extracellular Protein A (ESXA) Antibody (FITC)
from Abbexa
20-abx300381 | [ 100 ug: 411.00 EUR ] [ 1 mg: 1845.00 EUR ] [ 200 ug: 599.00 EUR ] [ 20 ug: 182.00 EUR ] [ 50 ug: 300.00 EUR ]
ESAT-6 Secretion System Extracellular Protein A (ESXA) Antibody (Biotin)
from Abbexa
20-abx300382 | [ 100 ug: 411.00 EUR ] [ 1 mg: 1845.00 EUR ] [ 200 ug: 599.00 EUR ] [ 20 ug: 182.00 EUR ] [ 50 ug: 300.00 EUR ]
Mycobacterium tuberculosis ESAT-6 like protein EsxG Control/blocking peptide
from Alpha Diagnostics
AB-23071-P | 100ug: 164.00 EUR
ESAT sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)
from ABM
K0696005 | 3 x 1.0 ug: 376.00 EUR
Recombinant purified (E. coli) Mycobacterium tuberculosis antigen (6kDa/ESAT-6)
from Alpha Diagnostics
MTB06-R | 100 ug: 773.00 EUR
Recombinant Mycobacterium Tuberculosis ESAT-6 Protein (aa 1-99) [His]
from Creative Biolabs
VAng-Lsx06137-100g | 100 µg: 598.00 EUR
Description: Mycobacterium Tuberculosis (H37Rv) ESAT-6, recombinant protein from E. coli.
Recombinant Mycobacterium Tuberculosis ESAT-6 Protein (aa 1-99) [His]
from Creative Biolabs
VAng-Lsx06137-1mg | 1 mg: 3072.00 EUR
Description: Mycobacterium Tuberculosis (H37Rv) ESAT-6, recombinant protein from E. coli.
ESAT Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-C-term-HA)
from ABM
LV724668 | 1.0 ug DNA: Ask for price
ESAT Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)
from ABM
LV724669 | 1.0 ug DNA: Ask for price
ESAT Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)
from ABM
LV724670 | 1.0 ug DNA: Ask for price
ESAT sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1)
from ABM
K0696006 | 1.0 ug DNA: 167.00 EUR
ESAT sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2)
from ABM
K0696007 | 1.0 ug DNA: 167.00 EUR
ESAT sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3)
from ABM
K0696008 | 1.0 ug DNA: 167.00 EUR
Recombinant purified M. tuberculosis antigen (6kDa/ESAT-6) control for Western
from Alpha Diagnostics
MTB061-C | 100 ul: 286.00 EUR
Mouse Anti-M.Tuberculosis 6kDa/ESAT-6 IgG ELISA kit, 96 tests, Quantitative
from Alpha Diagnostics
990-230-06G | 1 Kit: 773.00 EUR
Rabbit Anti-Mycobacterium tuberculosis ESAT-6 like protein EsxG IgG (aff pure)
from Alpha Diagnostics
AB-23071-A | 100ug: 482.00 EUR
Recombinant M. tuberculosis ESAT-6-like protein EsxB Protein, His, Yeast-100ug
from EnQuireBio
QP8759-ye-100ug | 100ug: 788.00 EUR
Recombinant M. tuberculosis ESAT-6-like protein EsxB Protein, His, Yeast-10ug
from EnQuireBio
QP8759-ye-10ug | 10ug: 362.00 EUR
Recombinant M. tuberculosis ESAT-6-like protein EsxB Protein, His, Yeast-1mg
from EnQuireBio
QP8759-ye-1mg | 1mg: 2747.00 EUR
Recombinant M. tuberculosis ESAT-6-like protein EsxB Protein, His, Yeast-200ug
from EnQuireBio
QP8759-ye-200ug | 200ug: 1260.00 EUR
Recombinant M. tuberculosis ESAT-6-like protein EsxB Protein, His, Yeast-500ug
from EnQuireBio
QP8759-ye-500ug | 500ug: 1804.00 EUR
Recombinant M. tuberculosis ESAT-6-like protein EsxB Protein, His, Yeast-50ug
from EnQuireBio
QP8759-ye-50ug | 50ug: 480.00 EUR
Mouse Anti-M. Tuberculosis 6kDa/ESAT-6 IgM ELISA kit, 96 tests, Quantitative
from Alpha Diagnostics
990-235-06M | 1 Kit: 773.00 EUR
Recombinant M. tuberculosis ESAT-6-like protein EsxH Protein, His-SUMO, E.coli-100ug
from EnQuireBio
QP7384-ec-100ug | 100ug: 707.00 EUR
Recombinant M. tuberculosis ESAT-6-like protein EsxH Protein, His-SUMO, E.coli-10ug
from EnQuireBio
QP7384-ec-10ug | 10ug: 326.00 EUR
Recombinant M. tuberculosis ESAT-6-like protein EsxH Protein, His-SUMO, E.coli-1mg
from EnQuireBio
QP7384-ec-1mg | 1mg: 2303.00 EUR
Recombinant M. tuberculosis ESAT-6-like protein EsxH Protein, His-SUMO, E.coli-200ug
from EnQuireBio
QP7384-ec-200ug | 200ug: 1115.00 EUR
Recombinant M. tuberculosis ESAT-6-like protein EsxH Protein, His-SUMO, E.coli-500ug
from EnQuireBio
QP7384-ec-500ug | 500ug: 1514.00 EUR
Recombinant M. tuberculosis ESAT-6-like protein EsxH Protein, His-SUMO, E.coli-50ug
from EnQuireBio
QP7384-ec-50ug | 50ug: 435.00 EUR
Rabbit Anti-M. Tuberculosis antigens (6Kda/ESAT+16kDa+38KDa/Ag85b mixture of proteins antiserum
from Alpha Diagnostics
MTB6381-S | 100 ul: 457.00 EUR
Recombinant purified ESAT-6 (6 kDa early secretory antigen of T cells; Mycobacterium Tuberculosis)
from Alpha Diagnostics
RP-977 | 10 ug: 225.00 EUR
Recombinant purified ESAT-6 (6 kDa early secretory antigen of T cells; Mycobacterium Tuberculosis)
from Alpha Diagnostics
RP-977-100 | 100 ug: 773.00 EUR
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