ESAT 6 like protein EsxO

Improved outcomes for index cholecystectomy for acute cholecystitis following a dedicated emergency surgery and trauma service (ESAT).

Journal: European journal of trauma and emergency surgery : official publication of the European Trauma Society

Februar/5/2020
Early laparoscopic cholecystectomy (ELC) has shown to reduce length of stay and improve patients’ satisfaction as compared to delayed laparoscopic cholecystectomy (DLC). However, logistics and manpower limitations often preclude ELC.A retrospective study was conducted in a single institute to compare outcomes of AC before (August 2013-2014) and after (August 2017-2018) establishment of emergency surgery and trauma (ESAT).There were 82 patients in pre-ESAT period and 172 patients in ESAT period. Mean age was 52.3 ± 11.6 and 55.7 ± 13.8 years, respectively, p = 0.369. There were more patients with moderate-severe grading of cholecystitis based on Tokyo Guidelines (TG 18) in ESAT 143/172 (83.1%) as compared to pre-ESAT 65/82 (79.3%), p = 0.042. Index cholecystectomy was performed in 145/172 (84.3%) of patients in the ESAT vs 34/82 (41.5%) of patients in the pre-ESAT period (p = 0.001). Time interval between booking to surgery was 180 ± 56 min in ESAT vs 197 ± 98 min in pre-ESAT, p = 0.014. Operative duration was shorter in ESAT 121 ± 38.5 min vs 139 ± 53.4, in pre-ESAT period, p = 0.030. Conversion rates were lower in ESAT (4/172, 2.3%) vs (9/72, 11%) in pre-ESAT, p = 0.003. Length of stay was shorter in ESAT (DLC 1.89 ± 1.6 and ELC ± 2.9 days) as compared to pre-ESAT (DLC 4.55 ± 2.2 and ELC 5.03 ± 2.6 days), p = 0.001. 30-day readmissions were lower in ESAT (3/172, 1.7%) vs pre-ESAT (8/72, 9.8%).The ESAT model provided more early laparoscopic cholecystectomies with improved efficiency and clinical outcomes.
Si Goh; Clement Chia; Jing Ong; John Quek; Woan Lim; Kok Tan; Jerry Goo

EspM Is a Conserved Transcription Factor That Regulates Gene Expression in Response to the ESX-1 System.

Journal: mBio

Februar/5/2020
Pathogenic mycobacteria encounter multiple environments during macrophage infection. Temporally, the bacteria are engulfed into the phagosome, lyse the phagosomal membrane, and interact with the cytosol before spreading to another cell. Virulence factors secreted by the mycobacterial ESX-1 (ESAT-6-system-1) secretion system mediate the essential transition from the phagosome to the cytosol. It was recently discovered that the ESX-1 system also regulates mycobacterial gene expression in Mycobacterium marinum (R. E. Bosserman, T. T. Nguyen, K. G. Sanchez, A. E. Chirakos, et al., Proc Natl Acad Sci U S A 114:E10772-E10781, 2017, https://doi.org/10.1073/pnas.1710167114), a nontuberculous mycobacterial pathogen, and in the human-pathogenic species M. tuberculosis (A. M. Abdallah, E. M. Weerdenburg, Q. Guan, R. Ummels, et al., PLoS One 14:e0211003, 2019, https://doi.org/10.1371/journal.pone.0211003). It is not known how the ESX-1 system regulates gene expression. Here, we identify the first transcription factor required for the ESX-1-dependent transcriptional response in pathogenic mycobacteria. We demonstrate that the gene divergently transcribed from the whiB6 gene and adjacent to the ESX-1 locus in mycobacterial pathogens encodes a conserved transcription factor (MMAR_5438, Rv3863, now espM). We prove that EspM from both M. marinum and M. tuberculosis directly and specifically binds the whiB6-espM intergenic region. We show that EspM is required for ESX-1-dependent repression of whiB6 expression and for the regulation of ESX-1-associated gene expression. Finally, we demonstrate that EspM functions to fine-tune ESX-1 activity in M. marinum Taking the data together, this report extends the esx-1 locus, defines a conserved regulator of the ESX-1 virulence pathway, and begins to elucidate how the ESX-1 system regulates gene expression.IMPORTANCE Mycobacterial pathogens use the ESX-1 system to transport protein substrates that mediate essential interactions with the host during infection. We previously demonstrated that in addition to transporting proteins, the ESX-1 secretion system regulates gene expression. Here, we identify a conserved transcription factor that regulates gene expression in response to the ESX-1 system. We demonstrate that this transcription factor is functionally conserved in M. marinum, a pathogen of ectothermic animals; M. tuberculosis, the human-pathogenic species that causes tuberculosis; and M. smegmatis, a nonpathogenic mycobacterial species. These findings provide the first mechanistic insight into how the ESX-1 system elicits a transcriptional response, a function of this protein transport system that was previously unknown.
Kevin Sanchez; Micah Ferrell; Alexandra Chirakos; Kathleen Nicholson; Robert Abramovitch; Matthew Champion; Patricia Champion

TB ESAT from EnoGene
E61H01301 | 100ug: 431.25 EUR
Recombinant TB esat-6 from MyBiosource
MBS8506624-1mg | 1mg: 510.00 EUR
Recombinant TB esat-6 from MyBiosource
MBS8506624-5x1mg | 5x1mg: 2140.00 EUR
PepPool: TB (ESAT-6), scanning from Mabtech AB
3624-1 | One vial with 25 ug of each peptide: 464.80 EUR
Description: Human
M. Tuberculosis ESAT-6 Antigen from Creative Diagnostics
DAGA-187 | 100 µg: 665.44 EUR
Description: Recombinant
ESAT Recombinant Protein (Human) from ABM
RP057123 | 100 ug: Ask for price
TB-16&TB-38 & ESAT from EnoGene
E61H01303 | 100ug: 255.00 EUR
Recombinant TB-16 & 38 & esat-6 from MyBiosource
MBS8506856-01mg | 0.1mg: 325.00 EUR
Recombinant TB-16 & 38 & esat-6 from MyBiosource
MBS8506856-5x01mg | 5x0.1mg: 1320.00 EUR
ESAT ORF Vector (Human) (pORF) from ABM
ORF019042 | 1.0 ug DNA: Ask for price
Recombinant Mycobacterium tuberculosis ESAT-6 from Creative Diagnostics
DAGH171 | 1 mg: 630.00 EUR
Description: Recombinant
ESAT-6-like protein EsxB Antibody from Abbexa
20-abx110662 | [ 20 ug: Ask for price ] [ 50 ug: Ask for price ] [ 100 ug: Ask for price ] [ 200 ug: Ask for price ] [ 1 mg: Ask for price ]
ESAT-6-like protein EsxB Antibody (HRP) from Abbexa
20-abx109228 | [ 20 ug: Ask for price ] [ 50 ug: Ask for price ] [ 100 ug: Ask for price ] [ 200 ug: Ask for price ] [ 1 mg: Ask for price ]
ESAT-6-Like Protein EsxB (esxB) Antibody from Abbexa
abx110662-100l | 100 µl: 162.50 EUR
ESAT-6-Like Protein EsxH (esxH) Antibody from Abbexa
abx140580-100g | 100 µg: 350.00 EUR
ESAT-6-like protein EsxB Antibody (FITC) from Abbexa
20-abx107810 | [ 20 ug: Ask for price ] [ 50 ug: Ask for price ] [ 100 ug: Ask for price ] [ 200 ug: Ask for price ] [ 1 mg: Ask for price ]
ESAT-6-like protein EsxB Antibody (Biotin) from Abbexa
20-abx106398 | [ 20 ug: Ask for price ] [ 50 ug: Ask for price ] [ 100 ug: Ask for price ] [ 200 ug: Ask for price ] [ 1 mg: Ask for price ]
ESAT sgRNA CRISPR Lentivector set (Human) from ABM
K0696001 | 3 x 1.0 ug: 406.80 EUR
ESAT 3'UTR GFP Stable Cell Line from ABM
TU057053 | 1.0 ml: 2799.60 EUR
ESAT-6-Like Protein EsxB (esxB) Antibody (HRP) from Abbexa
abx109228-100l | 100 µl: 162.50 EUR
ESAT-6-Like Protein EsxB (esxB) Antibody (FITC) from Abbexa
abx107810-100g | 100 µg: 362.50 EUR
ESAT-6-Like Protein EsxB (esxB) Antibody (FITC) from Abbexa
abx107810-20g | 20 µg: 162.50 EUR
ESAT-6-Like Protein EsxB (esxB) Antibody (FITC) from Abbexa
abx107810-50g | 50 µg: 250.00 EUR
Mycobacterium tuberculosis antigen EsaT-6 (Rv3875) Antibody from Abbexa
abx140048-01mg | 0.1 mg: 510.00 EUR
Mycobacterium tuberculosis antigen EsaT-6 (Rv3875) Antibody from Abbexa
abx140048-100g | 100 µg: 350.00 EUR
ESAT-6-Like Protein EsxB (esxB) Antibody (Biotin) from Abbexa
abx106398-100g | 100 µg: 362.50 EUR
ESAT-6-Like Protein EsxB (esxB) Antibody (Biotin) from Abbexa
abx106398-20g | 20 µg: 162.50 EUR
ESAT-6-Like Protein EsxB (esxB) Antibody (Biotin) from Abbexa
abx106398-50g | 50 µg: 250.00 EUR
ESAT Protein Vector (Human) (pPM-C-HA) from ABM
PV076167 | 500 ng: Ask for price
ESAT Protein Vector (Human) (pPB-C-His) from ABM
PV076165 | 500 ng: Ask for price
ESAT Protein Vector (Human) (pPB-N-His) from ABM
PV076166 | 500 ng: Ask for price
ESAT Protein Vector (Human) (pPM-C-His) from ABM
PV076168 | 500 ng: Ask for price
ESAT 3'UTR Luciferase Stable Cell Line from ABM
TU007053 | 1.0 ml: 2799.60 EUR
ESAT sgRNA CRISPR Lentivector (Human) (Target 1) from ABM
K0696002 | 1.0 ug DNA: 184.80 EUR
ESAT sgRNA CRISPR Lentivector (Human) (Target 2) from ABM
K0696003 | 1.0 ug DNA: 184.80 EUR
ESAT sgRNA CRISPR Lentivector (Human) (Target 3) from ABM
K0696004 | 1.0 ug DNA: 184.80 EUR
Mycobacterium tuberculosis ESAT-6-like protein EsxB (esxB) from Cusabio
1-CSB-EP363658MVZ | [ 10ug: Ask for price ] [ 50ug: Ask for price ] [ 100ug: Ask for price ] [ 200ug: Ask for price ] [ 500ug: Ask for price ] [ 1MG: Ask for price ]
Description: Recombinant Mycobacterium tuberculosis ESAT-6-like protein EsxB(esxB) expressed in E.coli
Mycobacterium tuberculosis ESAT-6-like protein EsxH (esxH) from Cusabio
1-CSB-EP363659MVZ | [ 10ug: Ask for price ] [ 50ug: Ask for price ] [ 100ug: Ask for price ] [ 200ug: Ask for price ] [ 500ug: Ask for price ] [ 1MG: Ask for price ]
Description: Recombinant Mycobacterium tuberculosis ESAT-6-like protein EsxH(esxH),partial expressed in E.coli
Mycobacterium tuberculosis ESAT-6-like protein esxB (esxB) from Cusabio
1-CSB-YP363658MVZ | [ 10ug: Ask for price ] [ 50ug: Ask for price ] [ 100ug: Ask for price ] [ 200ug: Ask for price ] [ 500ug: Ask for price ] [ 1MG: Ask for price ]
Description: Recombinant Mycobacterium tuberculosis ESAT-6-like protein esxB(esxB) expressed in Yeast
Recombinant Mycobacteroides abscessus ESAT-6-like protein (esxT) from Cusabio
CSB-EP4812GTR | 11120 mg: Ask for price
Recombinant Mycobacteroides abscessus ESAT-6-like protein (esxT) from Cusabio
CSB-EP4812GTR-100ug | 100ug: Ask for price
Description: 1-96aa
Recombinant Mycobacteroides abscessus ESAT-6-like protein (esxT) from Cusabio
CSB-EP4812GTR-1mg | 1mg: Ask for price
Description: 1-96aa
Recombinant Mycobacteroides abscessus ESAT-6-like protein (esxT) from Cusabio
CSB-EP4812GTR-20ug | 20ug: Ask for price
Description: 1-96aa
Recombinant Mycobacteroides abscessus ESAT-6-like protein (D2E76_02620) from Cusabio
CSB-EP4811GTR | 11119 mg: Ask for price
Recombinant Mycobacteroides abscessus ESAT-6-like protein (D2E76_02620) from Cusabio
CSB-EP4811GTR-100ug | 100ug: Ask for price
Description: 1-92aa
Recombinant Mycobacteroides abscessus ESAT-6-like protein (D2E76_02620) from Cusabio
CSB-EP4811GTR-1mg | 1mg: Ask for price
Description: 1-92aa
Recombinant Mycobacteroides abscessus ESAT-6-like protein (D2E76_02620) from Cusabio
CSB-EP4811GTR-20ug | 20ug: Ask for price
Description: 1-92aa
Recombinant Mycobacterium tuberculosis ESAT-6-like protein EsxH from MyBiosource
MBS1103807-002mgEColi | 0.02mg(E-Coli): 375.00 EUR
Recombinant Mycobacterium tuberculosis ESAT-6-like protein EsxH from MyBiosource
MBS1103807-01mgEColi | 0.1mg(E-Coli): 635.00 EUR
Recombinant Mycobacterium tuberculosis ESAT-6-like protein EsxH from MyBiosource
MBS1103807-1mgEColi | 1mg(E-Coli): 1925.00 EUR
Recombinant Mycobacterium tuberculosis ESAT-6-like protein EsxH from MyBiosource
MBS1103807-5x1mgEColi | 5x1mg(E-Coli): 8405.00 EUR
Recombinant Mycobacterium tuberculosis ESAT-6-like protein EsxH from MyBiosource
MBS9421049-002mg | 0.02mg: 460.00 EUR
Recombinant Mycobacterium tuberculosis ESAT-6-like protein EsxH from MyBiosource
MBS9421049-01mg | 0.1mg: 795.00 EUR
Recombinant Mycobacterium tuberculosis ESAT-6-like protein EsxH from MyBiosource
MBS9421049-1mg | 1mg: 2525.00 EUR
Recombinant Mycobacterium tuberculosis ESAT-6-like protein EsxH from MyBiosource
MBS9421049-5x1mg | 5x1mg: 11205.00 EUR
Rabbit Anti-Mycobacterium tuberculosis EsaT-6 Polyclonal Antibody from BioVenic
VD7N135 | 1 Each: Ask for price
ESAT-6 Secretion System Extracellular Protein A (esxA) Antibody from Abbexa
abx300131-100g | 100 µg: 362.50 EUR
ESAT-6 Secretion System Extracellular Protein A (ESXA) Antibody from Abbexa
20-abx300131 | [ 20 ug: Ask for price ] [ 50 ug: Ask for price ] [ 100 ug: Ask for price ] [ 200 ug: Ask for price ] [ 1 mg: Ask for price ]
ESAT-6 Secretion System Extracellular Protein A (esxA) Antibody from Abbexa
abx300131-20g | 20 µg: 162.50 EUR
ESAT-6 Secretion System Extracellular Protein A (esxA) Antibody from Abbexa
abx300131-50g | 50 µg: 250.00 EUR
Recombinant Mycobacterium bovis ESAT-6-like protein esxH (esxH) from MyBiosource
MBS1152262-002mgBaculovirus | 0.02mg(Baculovirus): 1020.00 EUR
Recombinant Mycobacterium bovis ESAT-6-like protein esxH (esxH) from MyBiosource
MBS1152262-002mgEColi | 0.02mg(E-Coli): 590.00 EUR
Recombinant Mycobacterium bovis ESAT-6-like protein esxH (esxH) from MyBiosource
MBS1152262-002mgYeast | 0.02mg(Yeast): 770.00 EUR
Recombinant Mycobacterium bovis ESAT-6-like protein esxH (esxH) from MyBiosource
MBS1152262-01mgEColi | 0.1mg(E-Coli): 700.00 EUR
Recombinant Mycobacterium bovis ESAT-6-like protein esxH (esxH) from MyBiosource
MBS1152262-01mgYeast | 0.1mg(Yeast): 905.00 EUR
Recombinant Mycobacterium bovis ESAT-6-like protein esxM (esxM) from MyBiosource
MBS1235169-002mgBaculovirus | 0.02mg(Baculovirus): 1020.00 EUR
Recombinant Mycobacterium bovis ESAT-6-like protein esxM (esxM) from MyBiosource
MBS1235169-002mgEColi | 0.02mg(E-Coli): 595.00 EUR
Recombinant Mycobacterium bovis ESAT-6-like protein esxM (esxM) from MyBiosource
MBS1235169-002mgYeast | 0.02mg(Yeast): 775.00 EUR
Recombinant Mycobacterium bovis ESAT-6-like protein esxM (esxM) from MyBiosource
MBS1235169-01mgEColi | 0.1mg(E-Coli): 705.00 EUR
Recombinant Mycobacterium bovis ESAT-6-like protein esxM (esxM) from MyBiosource
MBS1235169-01mgYeast | 0.1mg(Yeast): 905.00 EUR
Recombinant Mycobacterium bovis ESAT-6-like protein esxR (esxR) from MyBiosource
MBS1242423-002mgBaculovirus | 0.02mg(Baculovirus): 1020.00 EUR
Recombinant Mycobacterium bovis ESAT-6-like protein esxR (esxR) from MyBiosource
MBS1242423-002mgEColi | 0.02mg(E-Coli): 590.00 EUR
Recombinant Mycobacterium bovis ESAT-6-like protein esxR (esxR) from MyBiosource
MBS1242423-002mgYeast | 0.02mg(Yeast): 775.00 EUR
Recombinant Mycobacterium bovis ESAT-6-like protein esxR (esxR) from MyBiosource
MBS1242423-01mgEColi | 0.1mg(E-Coli): 700.00 EUR
Recombinant Mycobacterium bovis ESAT-6-like protein esxR (esxR) from MyBiosource
MBS1242423-01mgYeast | 0.1mg(Yeast): 905.00 EUR
Recombinant Mycobacterium bovis ESAT-6-like protein esxB (esxB) from MyBiosource
MBS1159977-002mgBaculovirus | 0.02mg(Baculovirus): 1020.00 EUR
Recombinant Mycobacterium bovis ESAT-6-like protein esxB (esxB) from MyBiosource
MBS1159977-002mgEColi | 0.02mg(E-Coli): 595.00 EUR
Recombinant Mycobacterium bovis ESAT-6-like protein esxB (esxB) from MyBiosource
MBS1159977-002mgYeast | 0.02mg(Yeast): 775.00 EUR
Recombinant Mycobacterium bovis ESAT-6-like protein esxB (esxB) from MyBiosource
MBS1159977-01mgEColi | 0.1mg(E-Coli): 690.00 EUR
Recombinant Mycobacterium bovis ESAT-6-like protein esxB (esxB) from MyBiosource
MBS1159977-01mgYeast | 0.1mg(Yeast): 910.00 EUR
Recombinant Mycobacterium bovis ESAT-6-like protein esxN (esxN) from MyBiosource
MBS1131092-002mgBaculovirus | 0.02mg(Baculovirus): 1015.00 EUR
Recombinant Mycobacterium bovis ESAT-6-like protein esxN (esxN) from MyBiosource
MBS1131092-002mgEColi | 0.02mg(E-Coli): 590.00 EUR
Recombinant Mycobacterium bovis ESAT-6-like protein esxN (esxN) from MyBiosource
MBS1131092-002mgYeast | 0.02mg(Yeast): 770.00 EUR
Recombinant Mycobacterium bovis ESAT-6-like protein esxN (esxN) from MyBiosource
MBS1131092-01mgEColi | 0.1mg(E-Coli): 700.00 EUR
Recombinant Mycobacterium bovis ESAT-6-like protein esxN (esxN) from MyBiosource
MBS1131092-01mgYeast | 0.1mg(Yeast): 900.00 EUR
Recombinant Mycobacterium bovis ESAT-6-like protein esxI (esxI) from MyBiosource
MBS1126020-002mgBaculovirus | 0.02mg(Baculovirus): 1015.00 EUR
Recombinant Mycobacterium bovis ESAT-6-like protein esxI (esxI) from MyBiosource
MBS1126020-002mgEColi | 0.02mg(E-Coli): 590.00 EUR
Recombinant Mycobacterium bovis ESAT-6-like protein esxI (esxI) from MyBiosource
MBS1126020-002mgYeast | 0.02mg(Yeast): 770.00 EUR