Recently, DNA methylation evaluation of FAM19A4 in cervical scrapes has been proven to adequately detect high-grade cervical intraepithelial neoplasia and cervical most cancers (≥ CIN3) in high-risk HPV (hrHPV)-positive women. Here, we in contrast the medical performance of FAM19A4 methylation evaluation to cytology and HPV16/18 genotyping, individually and in mixture, for ≥ CIN3 detection in hrHPV-positive women collaborating in a potential observational multi-center cohort research.
The research population comprised hrHPV-positive women aged 18-66 years, visiting a gynecological outpatient clinic. From these women, cervical scrapes and colposcopy-directed biopsies (for histological affirmation) had been obtained. Cervical scrapes had been analyzed for FAM19A4 gene promoter methylation, cytology and HPV16/18 genotyping. Both sensitivity and specificity of FAM19A4 methylation evaluation had been related to age.
Methylation evaluation was carried out by quantitative methylation-specific PCR (qMSP). Sensitivities and specificities for ≥ CIN3 had been in contrast between exams. Stratified analyses had been carried out for variables that probably affect marker performance. Of all 508 hrHPV-positive women, the sensitivities for ≥ CIN3 of cytology, FAM19A4 methylation evaluation, and cytology mixed with HPV16/18 genotyping had been 85.6, 75.6 and 92.2%, respectively, with corresponding specificities of 49.8, 71.1 and 29.4%, respectively.
In women ≥ 30 years (n = 287), ≥ CIN3 sensitivity of FAM19A4 methylation evaluation was 88.3% which was noninferior to that of cytology at a considerably larger specificity [62.1% (95%CI: 55.8-68.4) compared to 47.6%. In conclusion, among hrHPV-positive women from an outpatient population aged ≥ 30 years, methylation analysis of FAM19A4 is an attractive marker for the identification of women with ≥ CIN3.
Cytology has been the mainstay of cervical dysplasia and cancer screening in the United States. The specificity of a woman harboring a high-grade lesion when identified as high-grade squamous intraepithelial lesion on Pap test is high; however, the test suffers from low sensitivity. Epidemiology studies have demonstrated that human papillomavirus (HPV) types 16 and 18 account for most cervical squamous cell carcinomas. Tests have been developed to identify high-risk HPV, some specifically to identify HPV 16 and 18.
Young investigator challenge: Comparison of 2 different methods of manual slide screening in semiautomated gynecologic cytology.
Negative gynecologic cytology cases (ie, those diagnosed as negative for intraepithelial lesion or malignancy) are manually reviewed by 2 methods using semiautomated screening: 1) immediate full slide review (FSR) after fields-of-view analysis (FOV) (FOV + FSR), and 2) quality-assurance/high-risk, quintile-directed full manual review (FMR). Data supporting current guidelines were limited.
The authors investigated FMR, FOV + FSR, and the review process in general. Gynecologic cytology cases from 2009 to 2014 at Massachusetts General Hospital were analyzed. The data comprised 93,169 patients, 194,656 specimens, and 49,979 human papillomavirus (HPV) tests. Simultaneous to the increase in HPV detection methods, interdisciplinary groups are making recommendations on the managerial use of the tests.
In patients who underwent FMR, the epithelial cell abnormality (ECA) rate was correlated with the HPV-positive rate (correlation coefficient [r(2) ] = 0.82; Y = 0.19X + 0.02), and each charges decreased with age. For sufferers who underwent FOV + FSR, the ECA charge was additionally associated to the HPV-positive charge, and each charges decreased with age. The slope of the line evaluating the FMR ECA charge with the FMR HPV-positive charge could be a helpful surrogate marker of screening accuracy.
The FMR group had comparable HPV-positive charges in comparison with the FOV + FSR group (2%-52% vs 9%-68%, respectively). HPV-positive sufferers had a larger threat of ECA than HPV-negative sufferers (40% vs 8%; P < .0001). Currently, guide evaluation allocates sources inefficiently, to older, HPV-negative sufferers as in comparison with youthful, HPV-positive sufferers. FMR ought to be assigned based on HPV standing and age.
Additionally, too many sufferers who’ve HPV-positive specimens with ECA proceed from major screening to FMR. This imbalance might be noticed in the slope of the line evaluating the FMR ECA charge with the HPV-positive charge (which could function a surrogate marker for adequacy of FOV + FSR screening). Therefore, laboratories ought to attempt to scale back the quantity of HPV-positive circumstances that attain FMR. Current evaluation procedures commit too few sources to youthful sufferers.
Cytology for the detection of early recurrence of gynecologic most cancers in the vaginal vault.
The actual profit of follow-up cervical cytology in women handled for gynecological most cancers is unclear. This research was designed to evaluate the charge of success of cytological examinations in the detection of early vaginal recurrence of gynecological most cancers in women discovered by different strategies to have vaginal recurrence of cervical and endometrial most cancers. Records of cytological examinations.
Records from 1979 to 2010. Sixteen women (48.5%) had symptomatic vaginal recurrence related to distant metastases, whereas 17 (51.5%) had vaginal recurrence solely. Cytology was destructive in 12 women (36.4%) with each symptomatic and asymptomatic recurrence and optimistic in the different 21 (63.6%). In 9 of these 21 women (42.9%), the illness was restricted to the vaginal vault, whereas the remaining 12 (57.1%) introduced with vaginal lesions related to distant metastases. Cytology was optimistic in 9 of the 17 (52.9%) women whose recurrence was restricted to the vaginal vault and destructive in 8 (47.1%).
 Wright-Giemsa Solution |
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M1438-1000 |
Biovision |
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EUR 316 |
Wright-Giemsa Solution |
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M1438-500 |
Biovision |
|
EUR 207 |
 Wright-Giemsa Stain Kit |
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K1438-30 |
Biovision |
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EUR 262 |
Wright-Giemsa Stain Kit |
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K1438-500 |
Biovision |
|
EUR 370 |
 Antibody) Flap Endonuclease GEN Homolog 1 (GEN1) Antibody |
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abx034396-400ul |
Abbexa |
400 ul |
EUR 523 |
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Flap Endonuclease GEN Homolog 1 (GEN1) Antibody |
|
abx034396-80l |
Abbexa |
80 µl |
EUR 286 |
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Flap Endonuclease GEN Homolog 1 (GEN1) Antibody |
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20-abx135921 |
Abbexa |
-
EUR 411.00
-
EUR 592.00
-
EUR 182.00
-
EUR 314.00
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- 100 ul
- 200 ul
- 20 ul
- 50 ul
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 STAT Double Staining ragent for Double staining Mouse and Sheep antibodies, 450 Plus slides |
|
NB330DB |
Innovex |
25 ml |
EUR 740 |
 Prosi Silver Staining Kit for 25 mini gel |
|
S3200-020 |
GenDepot |
1kit |
EUR 361 |
 ?-Galactosidase Staining Kit |
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AKR-100 |
Cell Biolabs |
1 kit |
EUR 432 |
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Description: This simple assay measures the transfection efficiency of the LacZ gene using a simple staining protocol. |
 DAPI Staining Kit |
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abx097206-1Kit |
Abbexa |
1 Kit |
EUR 230 |
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 PI Staining Kit |
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abx097208-1Kit |
Abbexa |
1 Kit |
EUR 230 |
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 STAT Double Staining reagent for Double staining of Mouse and Rabbit antibodies, 450 plus slides |
|
NB326DB |
Innovex |
25 ml |
EUR 740 |
 STAT Double Staining Reagent for Double staining of Mouse and Goat antibodies, 450 plus slides |
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NB327DB |
Innovex |
25 ml |
EUR 740 |
 2019-nCoV IgG/IgM RapiCard InstaTest for Whole Blood/Serum/Plasma samples |
|
GEN-176552-25tests |
Cortez Diagnostic |
25 tests |
EUR 276 |
|
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus. |
2019-nCoV IgG/IgM RapiCard InstaTest for Whole Blood/Serum/Plasma samples |
|
GEN-176552-50tests |
Cortez Diagnostic |
50 tests |
EUR 472 |
|
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus. |
 2019-nCoV IgG/IgM RapiCard InstaTest Single use kit for Fingerstick Whole Blood samples |
|
GEN-176553-25tests |
Cortez Diagnostic |
25 tests |
EUR 275 |
|
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus. |
2019-nCoV IgG/IgM RapiCard InstaTest Single use kit for Fingerstick Whole Blood samples |
|
GEN-176553-50tests |
Cortez Diagnostic |
50 tests |
EUR 470 |
|
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus. |
 Ponceau S Staining Solution |
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PW005 |
Bio Basic |
100ml |
EUR 69.14 |
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 ProsiBlue Gel Staining Solution |
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P7300-050 |
GenDepot |
500ml |
EUR 174 |
 PI / RNase Staining Buffer |
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20-abx090619 |
Abbexa |
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 Hoechst 33342 Staining Kit |
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abx097207-1Kit |
Abbexa |
1 Kit |
EUR 230 |
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 Human Histochemistry Staining Kit |
|
20-abx299039 |
Abbexa |
-
EUR 871.00
-
EUR 1219.00
-
EUR 565.00
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- 150 tests
- 500 tests
- 50 tests
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 CometAssay Silver Staining Kit |
|
4254-200-K |
Biotechne |
200 Tests |
EUR 325.8 |
 Beta-Galactosidase Staining Kit |
|
K2182-250 |
ApexBio |
250 assays |
EUR 293 |
Ponceau S Staining Solution |
|
B7778-100000 |
ApexBio |
100 g |
EUR 163 |
Ponceau S Staining Solution |
|
B7778-50000 |
ApexBio |
50 g |
EUR 126 |
 Alkaline Phosphatase Staining Kit |
|
K2035-50 |
Biovision |
50 assays |
EUR 379 |
Beta-Galactosidase Staining Kit |
|
K802-250 |
Biovision |
|
EUR 294 |
 Mouse Flap endonuclease GEN homolog 1, Gen1 ELISA KIT |
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ELI-27974m |
Lifescience Market |
96 Tests |
EUR 865 |
 Human Flap endonuclease GEN homolog 1, GEN1 ELISA KIT |
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ELI-12540h |
Lifescience Market |
96 Tests |
EUR 824 |
 DAB Enhancer for ampliying DAB stains in IHC staining, 60ml |
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NB308 |
Innovex |
60 ml |
EUR 427 |
 DAB Enhancer for ampliying DAB stains in IHC staining, 30ml |
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NB308-30 |
Innovex |
30 ml |
EUR 299 |
 Holder for Plasmid Midi, Maxi and Maxi plus, Ion Exchange column |
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FAPDE-holder-for-ion-exchange |
Favorgen |
1 prep |
EUR 158 |
 7-AAD Viability Staining Solution |
|
EXB0026 |
ExBio |
400 tests |
EUR 76 |
 SafePinky DNA Gel Staining Solution |
|
S1000-050 |
GenDepot |
500ml |
EUR 131 |
SafePinky DNA Gel Staining Solution |
|
S1000-100 |
GenDepot |
2X500ml |
EUR 188 |
SafePinky DNA Gel Staining Solution |
|
S1000-200 |
GenDepot |
4X500ml |
EUR 280 |
 Nerve Terminal Staining Kit V |
|
70034 |
Biotium |
1SET |
EUR 509 |
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Description: Minimum order quantity: 1 unit of 1SET |
 Coomassie Blue Staining Destaining Solution |
|
AR0140 |
BosterBio |
1kit (for 10 assays to stain gels of 5 x 8.5cm) |
EUR 70 |
 Coomassie Blue Fast Staining Solution |
|
AR0170 |
BosterBio |
100mL(for 10 assays to stain gels of 5 x 8.5cm) |
EUR 80 |
 7-AAD Viability Staining Buffer |
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abx090617-100tests |
Abbexa |
100 tests |
EUR 217 |
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 Fluorescein Active Caspase Staining Kit |
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K2047-25 |
ApexBio |
25 assays |
EUR 238 |
 Red Active Caspase Staining Kit |
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K2052-100 |
ApexBio |
100 assays |
EUR 572 |
Red Active Caspase Staining Kit |
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K2052-25 |
ApexBio |
25 assays |
EUR 238 |
 Live-Dead Cell Staining Kit |
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K2081-100 |
ApexBio |
100 staining |
EUR 460 |
 Blue-Color AP Staining Kit |
|
AP100B-1 |
SBI |
50 Assays |
EUR 262 |
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 Dual- Color AP Staining Kit |
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AP100D-1 |
SBI |
100 Assays |
EUR 412 |
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 Red-Color AP Staining Kit |
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AP100R-1 |
SBI |
50 Assays |
EUR 262 |
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 DNA Ploidy Analysis Staining Kit |
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K1439-1 |
Biovision |
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EUR 588 |
DNA Ploidy Analysis Staining Kit |
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K1439-5 |
Biovision |
|
EUR 2002 |
Live-Dead Cell Staining Kit |
|
K501-100 |
Biovision |
|
EUR 468 |
 for nucleic acid staining) DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) for nucleic acid staining |
|
AR1176 |
BosterBio |
10mL(100 assys) |
EUR 65 |
DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) for nucleic acid staining |
|
AR1177 |
BosterBio |
50mL(500 assys) |
EUR 101 |
, 30ml) Black-DAB Substrate/Chromogen System for IHC-HRP staining (stable), 30ml |
|
NB319-SBB |
Innovex |
30 ml |
EUR 369 |
 SDS-Blue™ - Coomassie based solution for protein staining in SDS-PAGE |
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04-GSB |
Gentaur Genprice |
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|
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Description: SDS-Blue™ is an innovative patented formula, based on Coomassie blue, that comes in a convenient ready to use format for staining proteins in SDS-PAGE (sodium dodecyl sulphate–polyacrylamide gel electrophoresis). The formulation of SDS-Blue™ provides numerous advantages compared to the classic Coomassie staining or to other similar protein stains. SDS-Blue™ provides higher sensitivity, virtually no background and eliminates the need for destaining of the gel due to its high specificity and affinity to bind to protein only. Not only does SDS-Blue™ yield clear and sharp bands, but it also contains no methanol and acetic acid, making it non-hazardous, safe to handle and friendly to the environment when disposed of. Two other advantages that make SDS-Blue™ the better option is that it is not light sensitive and can be stored at ambient temperature for 24 months. And this provides a considerable convenience, especially to laboratories that need and keep big amount of protein staining solutions – no more jammed refrigerators, you can keep SDS-Blue™ wherever it is most convenient for You! |
, 70ml NEW) Black-DAB Substrate/Chromogen System for IHC-HRP staining (stable), 70ml NEW |
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NB319-BB70 |
Innovex |
70 ml |
EUR 485 |
 staining, 10ml) BCIP/NBT, permanent blue chromogen for immuno-alkaline phosphatase (IHC) staining, 10ml |
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NB321NBT |
Innovex |
10 ml |
EUR 188 |
 Human Type I Collagen Staining Kit |
|
9044 |
Chondrex |
1 kit |
EUR 338.55 |
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Description: Human Type I Collagen Staining Kit |
) Yeast Viability Staining Kit (Green/Red) |
|
31063-1 |
Biotium |
1kit |
EUR 472 |
|
Description: Minimum order quantity: 1 unit of 1kit |
) StemTAG Alkaline Phosphatase Staining Kit (Red) |
|
CBA-300 |
Cell Biolabs |
100 assays |
EUR 461 |
|
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Staining Kits provide reagents for monitoring alkaline phosphatase activity via immunocytochemistry staining. |
) StemTAG Alkaline Phosphatase Staining Kit (Purple) |
|
CBA-306 |
Cell Biolabs |
100 assays |
EUR 461 |
|
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Staining Kits provide reagents for monitoring alkaline phosphatase activity via immunocytochemistry staining. |
 Coomassie Brilliant Blue Fast Staining Solution |
|
abx090652-200ml |
Abbexa |
200 ml |
EUR 203 |
|
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 Coomassie Brilliant Blue Gel Staining Kit |
|
abx090653-1Kit |
Abbexa |
1 Kit |
EUR 189 |
|
|
 Hoechst 33342 / PI Double Staining Kit |
|
abx097209-1Kit |
Abbexa |
1 Kit |
EUR 244 |
|
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 Human FoxP3 Transcription Factor Staining Kit |
|
OKTA00001 |
Aviva Systems Biology |
KIT |
EUR 454 |
|
Description: Description of target: ;Species reactivity: ;Application: FC;Assay info: ;Sensitivity: |
Human FoxP3 Transcription Factor Staining Kit |
|
OKTA00004 |
Aviva Systems Biology |
KIT |
EUR 454 |
|
Description: Description of target: ;Species reactivity: ;Application: FC;Assay info: ;Sensitivity: |
 Fluorescein Active Caspase-12 Staining Kit |
|
K2046-25 |
ApexBio |
25 assays |
EUR 238 |
 Fluorescein Active Caspase-2 Staining Kit |
|
K2048-25 |
ApexBio |
25 assays |
EUR 238 |
 Fluorescein Active Caspase-3 Staining Kit |
|
K2049-25 |
ApexBio |
25 assays |
EUR 238 |
 Fluorescein Active Caspase-8 Staining Kit |
|
K2050-25 |
ApexBio |
25 assays |
EUR 238 |
 Fluorescein Active Caspase-9 Staining Kit |
|
K2051-100 |
ApexBio |
100 assays |
EUR 572 |
Fluorescein Active Caspase-9 Staining Kit |
|
K2051-25 |
ApexBio |
25 assays |
EUR 238 |
 Red Active Caspase-3 Staining Kit |
|
K2053-100 |
ApexBio |
100 assays |
EUR 572 |
Red Active Caspase-3 Staining Kit |
|
K2053-25 |
ApexBio |
25 assays |
EUR 238 |
 Red Active Caspase-8 Staining Kit |
|
K2054-100 |
ApexBio |
100 assays |
EUR 572 |
Red Active Caspase-8 Staining Kit |
|
K2054-25 |
ApexBio |
25 assays |
EUR 238 |
 Red Active Caspase-9 Staining Kit |
|
K2055-100 |
ApexBio |
100 assays |
EUR 572 |
Red Active Caspase-9 Staining Kit |
|
K2055-25 |
ApexBio |
25 assays |
EUR 238 |
Vaginal cytology yielded false-negative outcomes in nearly half of the women with vaginal recurrence of gynecological most cancers. Patents of strategies used for early prognosis and detection of immortalization of cervical most cancers are additionally reviewed in this text.
