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Cervical cancer screening by molecular Pap-transformation of gynecologic cytology.

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Cervical cancer screening by molecular Pap-transformation of gynecologic cytology.
Cervical cancer is one of the widespread cancers in girls accounting for 7.9% of all cancers. In India it’s the second commonest cancer in girls. The immortality of the cancer cell and the comparatively lengthy timeframe from acquisition of an infection to growth of cervical cancer was established.
As main developments like LBC, HPV testing have been launched within the current years, screening has taken a brand new avatar, the Molecular pap!! The goals of this research have been: To evaluate gynecologic cytology and irregular outcomes with respect to standard and LBC. To research the function of HPV cotesting and ancillary checks carried out, that’s, HPV CISH, and p16ink4a by IHC.
About 71 924 Conventional and LBC samples have been included from August 2009 to December 2017. Cases for HPV testing alongside the traditional smears have been 1539. HPV could be examined from the identical LBC vial because the pattern stays steady at room temperature for six weeks. HPV DNA PCR was carried out in our laboratory for High and Low danger genotypes.
Cytology findings have been additionally correlated with histology. Detection charge of SILs in LBC samples have been greater (2.20%). The commonest abnormality was LSIL in LBC and ASCUS in typical smears. LBC with HPV cotesting improves sensitivity and specificity and reduces ambiguous outcomes; permits higher compliance, as a adverse outcome of each checks permits sufferers to get screening each 5 years, thereby rising screening intervals, essential in a useful resource restricted state of affairs.

Touch imprint (TI) cytology of needle core biopsies (NCB) in pathology laboratories: A observe survey of contributors within the College of American Pathologists (CAP) Non Gynecologic Cytopathology (NGC) Education Program.

Intra-procedural evaluation of contact imprint (TI) cytology from needle core biopsies (NCB) is used to make sure pattern adequacy and to offer rapid prognosis in numerous settings. We aimed to survey laboratories for present practices on the use of cytology with NCB. A voluntary supplemental questionnaire together with questions on demographics, personnel concerned, websites, accessioning, and reporting was despatched with the College of American Pathologists (CAP) 2015 Non gynecologic Cytopathology Education Program to survey practices of cytologic evaluation of NCB.
Among 844 respondents, 403 (48%) carried out cytologic evaluation of NCB. Common physique websites included lung (94%; 368/392), liver (87%; 340/ 392), and lymph nodes/spleen (77%; 303/392). Most of the time, a pathologist was current on-site 75% (295/393) for adequacy evaluation which was often verbally reported to the supplier performing the process.
Specimens have been ready by cytotechnologists (50%; 193 of 388) or pathologists (45%; 176 of 388) by touching the core to the slide (50%; 196 of 390) and rolling the core on the slide (45%; 177/390). Among the respondents, 19% stated that cytotechnologists independently carried out rapid evaluation of TI of NCB. Most laboratories (69%; 264/384) evaluated air-dried slides with a modified Giemsa stain and rendered one TI/NCB mixed report (87%, 334/385).
Cervical cancer screening by molecular Pap-transformation of gynecologic cytology.

p16/Ki-67 dual-stained cytology for detecting cervical (pre)cancer in a HPV-positive gynecologic outpatient inhabitants.

Women who check optimistic for a high-risk sort of the human papillomavirus (HPV) require triage testing to determine these girls with cervical intraepithelial neoplasia grade Three or cancer (≥CIN3). Although Pap cytology is taken into account a gorgeous triage check, its applicability is hampered by its subjective nature. This research prospectively in contrast the scientific efficiency of p16/Ki-67 dual-stained cytology to that of Pap cytology, with or with out HPV16/18 genotyping, in high-risk HPV-positive girls visiting gynecologic outpatient clinics (n=446 and age 18-66 years).

From all girls, cervical scrapes (for Pap cytology, HPV16/18 genotyping, and p16/Ki-67 dual-stained cytology) and colposcopy-directed biopsies have been obtained. This is the primary survey carried out particularly to find out the observe of adequacy evaluation of TI of NCB. Cytotechnologists are usually not performing adequacy evaluation of TI with out pathologist oversight. A single report is often issued which incorporates the adequacy evaluation as a component of the ultimate report.

The sensitivity of p16/Ki-67 dual-stained cytology for ≥CIN3 (93.8%) did neither differ considerably from that of Pap cytology (87.7%; ratio 1.07 and 95% confidence interval (CI): 0.97-1.18) nor from that of Pap cytology mixed with HPV16/18 genotyping (95.1%; ratio 0.99 and 95% CI: 0.91-1.07). However, the specificity of p16/Ki-67 dual-stained cytology for ≥CIN3 (51.2%) was considerably greater than that of Pap cytology (44.9%; ratio 1.14 and 95% CI: 1.01-1.29) and Pap cytology mixed with HPV16/18 genotyping (25.8%; ratio 1.99 and 95% CI: 1.68-2.35).

After exclusion of girls who had been referred as a result of of irregular Pap cytology, the specificity of p16/Ki-67 dual-stained cytology for ≥CIN3 (56.7%) remained the identical, whereas that of Pap cytology (60.3%) elevated considerably, leading to the same specificity of each assays (ratio 0.94 and 95% CI: 0.83-1.07) on this sub-cohort. In abstract, p16/Ki-67 dual-stained cytology has a superb scientific efficiency and is an fascinating goal microscopy-based triage device for high-risk HPV-positive girls.

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Cyclin And CBS Domain Divalent Metal Cation Transport Mediator 1 (CNNM1) Antibody
20-abx147914
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Cyclin And CBS Domain Divalent Metal Cation Transport Mediator 2 (CNNM2) Antibody
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Cyclin And CBS Domain Divalent Metal Cation Transport Mediator 2 (CNNM2) Antibody
20-abx327220
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Cyclin And CBS Domain Divalent Metal Cation Transport Mediator 1 (CNNM1) Antibody
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Cyclin And CBS Domain Divalent Metal Cation Transport Mediator 1 (CNNM1) Antibody
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Cyclin And CBS Domain Divalent Metal Cation Transport Mediator 1 (CNNM1) Antibody
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Cyclin And CBS Domain Divalent Metal Cation Transport Mediator 1 (CNNM1) Antibody
abx311414-100g 100 µg
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Cyclin And CBS Domain Divalent Metal Cation Transport Mediator 1 (CNNM1) Antibody
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Cyclin And CBS Domain Divalent Metal Cation Transport Mediator 1 (CNNM1) Antibody
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Cyclin And CBS Domain Divalent Metal Cation Transport Mediator 1 (CNNM1) Antibody (FITC)
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Cyclin And CBS Domain Divalent Metal Cation Transport Mediator 1 (CNNM1) Antibody (HRP)
abx311415-100g 100 µg
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Cyclin And CBS Domain Divalent Metal Cation Transport Mediator 1 (CNNM1) Antibody (HRP)
abx311415-20g 20 µg
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Cyclin And CBS Domain Divalent Metal Cation Transport Mediator 1 (CNNM1) Antibody (HRP)
abx311415-50g 50 µg
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Cyclin And CBS Domain Divalent Metal Cation Transport Mediator 1 (CNNM1) Antibody (FITC)
abx311416-100g 100 µg
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Cyclin And CBS Domain Divalent Metal Cation Transport Mediator 1 (CNNM1) Antibody (FITC)
abx311416-20g 20 µg
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Cyclin And CBS Domain Divalent Metal Cation Transport Mediator 1 (CNNM1) Antibody (FITC)
abx311416-50g 50 µg
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Universal Transport Medium Collection Kit - PK50
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Rat Cyclin And CBS Domain Divalent Metal Cation Transport Mediator 2 (CNNM2) ELISA Kit
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Cyclin And CBS Domain Divalent Metal Cation Transport Mediator 1 (CNNM1) Antibody (Biotin)
abx311417-100g 100 µg
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Cyclin And CBS Domain Divalent Metal Cation Transport Mediator 1 (CNNM1) Antibody (Biotin)
abx311417-20g 20 µg
EUR 162.5

Cyclin And CBS Domain Divalent Metal Cation Transport Mediator 1 (CNNM1) Antibody (Biotin)
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Cnnm4 (untagged) - Rat cyclin and CBS domain divalent metal cation transport mediator 4 (Cnnm4)
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Michel's Transport Fluid
MTF999 1000 ml
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SWA3364 1KIT
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HiCulture™ Transport Swab w/Selenite Med
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PBS Transport Tube 1ml
PBS1000-100 each
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PBS Transport Tube 1ml
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Sterile Viral Transport and Preservation Medium; Flat Bottom Tube (100 x 1 ml vials)
BSV-VTM-003 100 x 1 ml
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Description: Sterile Viral Transport and Preservation Medium; Flat Bottom Tube (100 x 1 ml vials)

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Description: Sterile Viral Transport and Preservation Medium; Flat Bottom Tube  (100 x 3 ml vials)

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UTM Transport Tubes - PK50
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Description: Sterile Viral Transport and Preservation Medium; Conical Bottom Tube  (500 x 3 ml vials)

Michel's Transport Wash Buffer
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Michel's Transport Wash Buffer
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Michel's Transport Wash Buffer
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Sterilin Transport Swabs - PK25
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HiCultureTM Transport Swabs w/
MS1062-100NO 1 unit
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Description: HiCultureTM Transport Swabs w/

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MS1062-10NO 1 unit
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Description: HiCultureTM Transport Swabs w/

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Description: HiCultureTM Transport Swabs w/

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Description: HiCultureTM Transport Swabs w/

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MS113-50NO 1 unit
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boite de 200 filtres pour cytologie, 24 x 76 mm + 1 trou 7 mm
CY430F2476/1T 200
EUR 13.1

boite de 200 filtres pour cytologie, 26 x 63 mm + 1 trou
CY430F2663/1T 200
EUR 13.1

Protein Transport Inhibitor MIX
E-CK-A013-200Tests 200 Tests
EUR 85

Protein Transport Inhibitor MIX
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Protein Transport Inhibitor MIX
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Cytological Sampling Brush - PK100
CYT1050 PK100
EUR 63.45

Swab Transport Amies Wire - PK500
SWA3020 PK500
EUR 2177.55

Fixative, for fixing cytological or hist
S102-500ML 1 unit
EUR 31.73
Description: Fixative, for fixing cytological or hist

DuraPorter Transport Box Red - EACH
STO0002 EACH
EUR 206.55

Sterile 5mL Transport Tube (Axygen)
TUBE5AX-S2 500box
EUR 267.6
Description: Skirted screw-cap 5mL Axygen tubes with caps. Sterile, RNase & DNase free.

Golgi Transport 1A (GOLT1A) Antibody
20-abx215662
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Golgi Transport 1A (GOLT1A) Antibody
20-abx325291
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Swab Transport Amies Plastic - PK500
SWA3016 PK500
EUR 473.85

Golgi Transport 1A (GOLT1A) Antibody
abx325291-100g 100 µg
EUR 250

Golgi Transport 1A (GOLT1A) Antibody
abx325291-50g 50 µg
EUR 187.5

Nuclear Transport Factor 2 Protein
20-abx260609
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HiCultureTM Transport Swabs w/ Cary -
MS202-100NO 1 unit
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HiCultureTM Transport Swabs w/ Cary -
MS202-10NO 1 unit
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HiCultureTM Transport Swabs w/ Cary -
MS202-50NO 1 unit
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Description: HiCultureTM Transport Swabs w/ Cary -

HiCultureTM Transport Swabs w/ CVTR
MS316-100NO 1 unit
EUR 46.04
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HiCultureTM Transport Swabs w/ CVTR
MS316-10NO 1 unit
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HiCultureTM Transport Swabs w/ CVTR
MS316-50NO 1 unit
EUR 27.62
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Swab Transport Amies Charcoal - PK500
SWA3018 PK500
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DuraPorter Transport Box Clear - EACH
STO0001 EACH
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Transport Tubes 10ml SIMPORT - PK1000
T55210ATTP PK1000
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Nuclear Transport Factor 2 Protein
abx260609-10g 10 µg
EUR 325

Nuclear Transport Factor 2 Protein
abx260609-2g 2 µg
EUR 225

HiCultureTM Transport Swabs w/ Amies
MS651-100NO 1 unit
EUR 46.04
Description: HiCultureTM Transport Swabs w/ Amies

HiCultureTM Transport Swabs w/ Amies
MS651-10NO 1 unit
EUR 9.27
Description: HiCultureTM Transport Swabs w/ Amies

HiCultureTM Transport Swabs w/ Amies
MS651-50NO 1 unit
EUR 27.62
Description: HiCultureTM Transport Swabs w/ Amies

HiCultureTM Transport Swabs w/ Amies
MS684-100NO 1 unit
EUR 46.04
Description: HiCultureTM Transport Swabs w/ Amies

HiCultureTM Transport Swabs w/ Amies
MS684-10NO 1 unit
EUR 9.27
Description: HiCultureTM Transport Swabs w/ Amies

Every gynecologic cytology specimen examined by each a CT and a CP from December 2004 to March 2015 was extracted from the laboratory info system; glandular interpretations have been excluded. Excel and SAS have been used for CT-CP pair evaluation. CT-CP pairs with fewer than 32 specimens (the bottom quartile) have been excluded. For the remaining CT-CP pairs, 30 specimens or 10% of the specimens (whichever was greater) have been randomly chosen for comparability by a weighted κ statistic. κ values larger than 0.6 represented good settlement inside CT-CP pairs.