Effect of Decellularization Parameters on the Efficient Production of Kidney Bioscaffolds.

The most preferred decellularization technique in creating bioscaffolds for complex organs such as kidneys is through detergent perfusion. Detergents such as sodium dodecyl sulfate (SDS) flow to the kidneys to remove cells but using this technique alone requires long treatment times. Coupling this technique with sonication treatment decreases decellularization time but may cause damages in the microarchitecture of the kidney.

This study evaluated the effects of decellularization parameters specifically SDS concentration (0.25%, 0.625%, and 1.0%wt/vol), flowrate (15, 30, and 45 mL/min), and sonicator power (0, 60, and 120 W) on the length of time needed to produce acellular and intact bioscaffolds. Decellularization was carried out by perfusing SDS to the renal artery of the cadaveric porcine kidney.

 while exposed to sonication treatment. Results showed that a significant decrease in decellularization time was observed in producing acellular scaffold when perfusion decellularization was coupled with sonication.

In addition, SDS concentration, SDS flowrate, and sonicator power had significant effects on the decellularization time while only sonicator power had a significant effect on the microarchitecture integrity of the scaffold. Lastly, H&E results showed that the produced bioscaffold showed complete cell removal with only minimal to moderate disruptions on the microarchitecture of the kidney.

Dog AGP ELISA Kit

E-40AGP 1 x 96 well plate
EUR 590.4

  Cow AGP ELISA kit

AGP-11 1x 96 wells plate
EUR 475

  Rat AGP ELISA kit

AGP-2 1x 96 wells plate
EUR 425

  Dog AGP ELISA kit

AGP-4 1x 96 wells plate
EUR 475

  Cat AGP ELISA kit

AGP-8 1x 96 wells plate
EUR 475

  Pig AGP ELISA kit

AGP-9 1x 96 wells plate
EUR 475

  Mouse AGP ELISA kit

AGP-1 1x 96 wells plate
EUR 475

  Monkey AGP ELISA kit

AGP-3 1x 96 wells plate
EUR 475

  Turkey AGP ELISA kit

AGP-6 1x 96 wells plate
EUR 450

  Chicken AGP ELISA kit

AGP-5 1x 96 wells plate
EUR 475

Human ?1-Acid glycoprotein,?1-AGP ELISA Kit

201-12-1776 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

anti-α1-acid glycoprotein (AGP)(29A1)

LF-MA0167 100 ul
EUR 400.8
Description: Mouse monoclonal to α1-acid glycoprotein (AGP)

Canine Alpha 1-Acid Glycoprotein (AGP) ELISA

GWB-374Z17 96 well Ask for price

Chicken Alpha 1-Acid Glycoprotein (AGP) ELISA

GWB-374Z11 96 well Ask for price

1 AGP acyltransferase 4 (AGPAT4) rabbit polyclonal antibody

AP17875PU-N 400 µl Ask for price

 Cow AGP SPARCL™ Assay

AGP-SP-11 1 kit
EUR 400

Rat AGP SPARCL™ Assay

AGP-SP-2 1 kit
EUR 500

 Cat AGP SPARCL™ Assay

AGP-SP-8 1 kit
EUR 500

Chicken AGP SPARCL™ Assay

AGP-SP-5 1 kit
EUR 500

CLIA kit for Rat ?1-AGP (?1-Acid Glycoprotein)

E-CL-R0695 1 plate of 96 wells
EUR 700.8
Description: A sandwich CLIA kit for quantitative measurement of Rat ?1-AGP (?1-Acid Glycoprotein) in samples from Serum, Plasma, Cell supernatant

CLIA kit for Mouse ?1-AGP (?1-Acid glycoprotein)

E-CL-M0687 1 plate of 96 wells
EUR 700.8
Description: A sandwich CLIA kit for quantitative measurement of Mouse ?1-AGP (?1-Acid glycoprotein) in samples from Serum, Plasma, Cell supernatant

AGP ELISA Kit (Bovine) (OKCD06846)

OKCD06846 96 Wells
EUR 1248
Description: Description of target: Functions as transport protein in the blood stream.UniRule annotation;Species reactivity: Bovine;Application: ELISA;Assay info: ;Sensitivity: < 7.0ng/mL

Human Alpha-1-Acid Glycoprotein ELISA Kit (AGP 1)

RK00779 96 Tests
EUR 625.2

Bovine Orosomucoid 1,ORM1/α1-AGP ELISA Kit

YLA0129BO-48T 48T Ask for price

Bovine Orosomucoid 1,ORM1/α1-AGP ELISA Kit

YLA0129BO-96T 96T Ask for price

Rabbit Orosomucoid 1,ORM1/α1-AGP ELISA Kit

YLA0199RB-48T 48T Ask for price

Rabbit Orosomucoid 1,ORM1/α1-AGP ELISA Kit

YLA0199RB-96T 96T Ask for price

Rat Alpha-1-Glycoprotein (α1-AGP) ELISA Kit

SL0760Ra -
EUR 498

Rabbit Orosomucoid 1, ORM1/α1-AGP GENLISA ELISA

KLX0128 1 x 96 wells
EUR 341

Bovine Orosomucoid 1, ORM1/α1-AGP GENLISA ELISA

KLB0201 1 x 96 wells
EUR 341

OPCD00959-10UG - AGP Recombinant Protein

OPCD00959-10UG 10ug
EUR 149

OPCD00959-50UG - AGP Recombinant Protein

OPCD00959-50UG 50ug
EUR 379

OPCD00959-200UG - AGP Recombinant Protein

OPCD00959-200UG 200ug
EUR 749

Rat α1-Acid glycoprotein(α1-AGP) ELISA Kit

CSB-E12730r-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Rat α1-Acid glycoprotein (α1-AGP) in samples from serum, plasma, urine, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Rat α1-Acid glycoprotein(α1-AGP) ELISA Kit

1-CSB-E12730r
  • Ask for price
  • Ask for price
  • Ask for price
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Rat α1-Acid glycoprotein(α1-AGP) in samples from serum, plasma, urine, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Cat AGP ELISA Kit | E-20AGP

E-20AGP Kit 1 x 96 well plate
EUR 760
Description:

Cat AGP ELISA Kit ; E-20AGP ; Immunology Consultants Laboratory

Species Reactivity: Cat

Format: Sandwich ELISA

Product Type: ELISA Kit

Range: 1.57 ng/ml - 100 ng/ml

Rat AGP ELISA Kit | E-25AGP

E-25AGP 1 x 96 well plate
EUR 680
Description:

Rat AGP ELISA Kit ; E-25AGP ; Immunology Consultants Laboratory

Species Reactivity: Rat

Format: Sandwich ELISA

Product Type: ELISA Kit

Range: 12.5 ng/ml - 400 ng/ml

Bovine α1-Acid glycoprotein, α1-AGP ELISA Kit

CSB-E08579b-24T 1 plate of 24 wells
EUR 198
Description: Quantitativecompetitive ELISA kit for measuring Bovine α1-Acid glycoprotein, α1-AGP in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Bovine α1-Acid glycoprotein, α1-AGP ELISA Kit

1-CSB-E08579b
  • Ask for price
  • Ask for price
  • Ask for price
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativecompetitive ELISA kit for measuring Bovine α1-Acid glycoprotein, α1-AGP in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Nori® Monkey AGP ELISA Kit

GR169206 96-well
EUR 461

Nori® Ferret AGP ELISA Kit

GR201130 96-well
EUR 461

Nori® Salmon AGP ELISA Kit

GR202130 96-well
EUR 461

Human alpha-1-Acid-Glycoprotein (Orosomucoid, AGP) Antibody

12011-05011 150 ug
EUR 175

Human alpha-1-Acid-Glycoprotein (Orosomucoid, AGP) Antibody

32220-05111 150 ug
EUR 313.2

Human AGP ELISA Kit | E-80AGP

E-80AGP 1 x 96 well plate
EUR 570
Description:

Human AGP ELISA Kit ; E-80AGP ; Immunology Consultants Laboratory

Species Reactivity: Human

Format: Sandwich ELISA

Product Type: ELISA Kit

Range: 5 ng/ml - 320 ng/ml

Nori® Hamster AGP ELISA Kit

GR172256 96-well
EUR 461

Monkey alpha-1-Acid-Glycoprotein (Orosomucoid, AGP) Antibody

36816-05111 150 ug
EUR 215

Bovine AGP ELISA Kit | E-10AGP

E-10AGP 1 x 96 well plate
EUR 712
Description:

Bovine AGP ELISA Kit ; E-10AGP ; Immunology Consultants Laboratory

Species Reactivity: Bovine

Format: Sandwich ELISA

Product Type: ELISA Kit

Range: 3.125 ng/ml - 100 ng/ml

ELISA kit for Rat ?1-AGP (Alpha-1-Acid Glycoprotein)

E-EL-R1081 1 plate of 96 wells
EUR 640.8
Description: A sandwich ELISA kit for quantitative measurement of Rat ?1-AGP (Alpha-1-Acid Glycoprotein) in samples from Serum, Plasma, Cell supernatant

Chicken AGP ELISA Kit | E-30AGP

E-30AGP 1 x 96 well plate
EUR 680
Description:

Chicken AGP ELISA Kit ; E-30AGP ; Immunology Consultants Laboratory

Species Reactivity: Chicken

Format: Sandwich ELISA

Product Type: ELISA Kit

Range: 6.25 ng/ml - 200 ng/ml

Nori® Zebrafish AGP ELISA Kit

GR206130 96-well
EUR 461

CLIA kit for Human ?1-AGP (Alpha-1-Acid Glycoprotein)

E-CL-H0001 1 plate of 96 wells
EUR 700.8
Description: A sandwich CLIA kit for quantitative measurement of Human ?1-AGP (Alpha-1-Acid Glycoprotein) in samples from Serum, Plasma, Cell supernatant

Human Alpha-1-Acid Glycoprotein(α 1-AGP) ELISA Kit

NSL1063Hu 96 wells
EUR 468

Bovine Alpha-1-Acid Glycoprotein(α 1-AGP) ELISA Kit

NSL1864Bo 96 T
EUR 528

Canine Alpha-1-Acid Glycoprotein(α 1-AGP) ELISA Kit

NSL2023Ca 2118 wells
EUR 528

Porcine Alpha-1-Acid Glycoprotein(α 1-AGP) ELISA Kit

NSL1417Po 96T
EUR 528

OKCD06846-96W - AGP ELISA Kit (Bovine)

OKCD06846-96W 96Wells
EUR 850

Rat Alpha-1-Acid Glycoprotein (α1-AGP)ELISA Kit

NSL1641r 96 Tests
EUR 498

Goat Alpha-1-Acid Glycoprotein (α1-AGP)ELISA Kit

NSL1164Gt 96T
EUR 528

Mouse Alpha-1-Acid Glycoprotein (α1-AGP)  ELISA Kit

NSL1616m 96 Test
EUR 528

Canine Alpha-1-Acid Glycoprotein (α1-AGP)ELISA Kit

NSL0049Ca 144 wells
EUR 528

Bovine Alpha-1-Acid glycoprotein (α1-AGP) ELISA Kit

RK00434 96 Tests
EUR 280

Chicken Alpha-1-Acid Glycoprotein (α1-AGP)ELISA Kit

NSL1202Ch 1297 T
EUR 528

Guinea pig Alpha-1-Acid Glycoprotein(α 1-AGP) ELISA Kit

NSL1468Gp 96T
EUR 528

Nori® Mouse Alpha 1 Acid Glycoprotein (AGP) ELISA Kit

GR117259 96-well
EUR 461

Nori® Rabbit α-1-acid glycoprotein (AGP) ELISA Kit

GR144192 96-well
EUR 461

Nori® Rat alpha-1-acid glycoprotein (AGP) ELISA Kit

GR118215 96-well
EUR 461

Nori® Human alpha-1-acid glycoprotein (AGP) ELISA Kit

GR111268 96-well
EUR 461

Nori® Sheep alpha-1-acid glycoprotein (AGP) ELISA Kit

GR116243 96-well
EUR 461

Nori® Equine alpha-1-acid Glycoprotein (AGP) ELISA Kit

GR106790 96-well
EUR 461

Nori® Canine alpha-1-acid glycoprotein (AGP) ELISA Kit

GR115288 96-well
EUR 461

Nori® Feline alpha-1-acid glycoprotein (AGP) ELISA Kit

GR188218 96-well
EUR 461

Nori® Bovine alpha-1-acid Glycoprotein (AGP) ELISA Kit

GR112256 96-well
EUR 461

Nori® Chicken alpha-1-acid glycoprotein (AGP) ELISA Kit

GR114102 96-well
EUR 461

Nori® Porcine alpha-1-acid glycoprotein (AGP) ELISA Kit

GR113282 96-well
EUR 461

Human Alpha-1-Acid Glycoprotein (Orosomucoid, AGP) AssayMax ELISA Kit

EG5101-1 96 Well Plate
EUR 475.2

Human alpha-1-Acid-Glycoprotein (Orosomucoid, AGP) Antibody (Biotin Conjugate)

12011-05021 150 ug
EUR 229

Human alpha-1-Acid-Glycoprotein (Orosomucoid, AGP) Antibody (Biotin Conjugate)

32220-05121 150 ug
EUR 442.8

Monkey alpha-1-Acid-Glycoprotein (Orosomucoid, AGP) Antibody (Biotin Conjugate)

36816-05121 150 ug
EUR 315

Nori® Guinea Pig alpha-1-acid Glycoprotein (AGP) ELISA Kit

GR171187 96-well
EUR 461

Rat α1-Acid glycoprotein(α1-AGP) Elisa Kit

EK720917 96 Wells
EUR 0.62

Rat α1-Acid glycoprotein(α1-AGP) ELISA Kit

EKC42303-48T 48T
EUR 474.04

Rat α1-Acid glycoprotein(α1-AGP) ELISA Kit

EKC42303-5x96T 5x96T
EUR 3216.7

Rat α1-Acid glycoprotein(α1-AGP) ELISA Kit

EKC42303-96T 96T
EUR 677.2

Rat α1-Acid glycoprotein,α1-AGP ELISA Kit

CN-01787R1 96T
EUR 567.6

Rat α1-Acid glycoprotein,α1-AGP ELISA Kit

CN-01787R2 48T
EUR 387.6

Rat α1-AGP(α1-Acid Glycoprotein) ELISA Kit

ER1432 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 3.75 ng/ml

Rat α1-Acid glycoprotein(α1-AGP)ELISA Kit

GA-E0093RT-48T 48T
EUR 380.4

Rat α1-Acid glycoprotein(α1-AGP)ELISA Kit

GA-E0093RT-96T 96T
EUR 595.2

Rat α1-Acid glycoprotein(α1-AGP)ELISA Kit

QY-E11560 96T
EUR 433.2

Rat α1-Acid glycoprotein,α1-AGP ELISA Kit

YLA0580RA-48T 48T Ask for price

Rat α1-Acid glycoprotein,α1-AGP ELISA Kit

YLA0580RA-96T 96T Ask for price

GOAT α1-Acid glycoprotein(α1-AGP)ELISA Kit

YLA0043GO-48T 48T Ask for price

GOAT α1-Acid glycoprotein(α1-AGP)ELISA Kit

YLA0043GO-96T 96T Ask for price

Mouse α1-Acid glycoprotein,α1-AGP Elisa Kit

EK730369 96 Wells
EUR 0.68

Human α1-Acid glycoprotein(α1-AGP) Elisa Kit

EK711545 96 Wells
EUR 0.18

Mouse α1-Acid glycoprotein,α1-AGP ELISA Kit

CN-02666M1 96T
EUR 556.8

Mouse α1-Acid glycoprotein,α1-AGP ELISA Kit

CN-02666M2 48T
EUR 375.6

Human α1-Acid glycoprotein(α1-AGP)ELISA Kit

GA-E1792HM-48T 48T
EUR 346.8

Human α1-Acid glycoprotein(α1-AGP)ELISA Kit

GA-E1792HM-96T 96T
EUR 559.2