Effect of Decellularization Parameters on the Efficient Production of Kidney Bioscaffolds.

The most preferred decellularization technique in creating bioscaffolds for complex organs such as kidneys is through detergent perfusion. Detergents such as sodium dodecyl sulfate (SDS) flow to the kidneys to remove cells but using this technique alone requires long treatment times. Coupling this technique with sonication treatment decreases decellularization time but may cause damages in the microarchitecture of the kidney.

This study evaluated the effects of decellularization parameters specifically SDS concentration (0.25%, 0.625%, and 1.0%wt/vol), flowrate (15, 30, and 45 mL/min), and sonicator power (0, 60, and 120 W) on the length of time needed to produce acellular and intact bioscaffolds. Decellularization was carried out by perfusing SDS to the renal artery of the cadaveric porcine kidney.

 while exposed to sonication treatment. Results showed that a significant decrease in decellularization time was observed in producing acellular scaffold when perfusion decellularization was coupled with sonication.

In addition, SDS concentration, SDS flowrate, and sonicator power had significant effects on the decellularization time while only sonicator power had a significant effect on the microarchitecture integrity of the scaffold. Lastly, H&E results showed that the produced bioscaffold showed complete cell removal with only minimal to moderate disruptions on the microarchitecture of the kidney.

Alpha1-AGP/ Rat Alpha1- AGP ELISA Kit
ELA-E0816r	Lifescience Market	96 Tests	EUR 886

Bovine AGP ELISA Kit
E-10AGP	Immunology Consultants Laboratory	1 x 96 well plate	EUR 461

Rat AGP ELISA Kit
E-25AGP	Immunology Consultants Laboratory	1 x 96 well plate	EUR 441

Chicken AGP ELISA Kit
E-30AGP	Immunology Consultants Laboratory	1 x 96 well plate	EUR 441

Dog AGP ELISA Kit
E-40AGP	Immunology Consultants Laboratory	1 x 96 well plate	EUR 492

Human AGP ELISA Kit
E-80AGP	Immunology Consultants Laboratory	1 x 96 well plate	EUR 370

Human ?1-Acid glycoprotein,?1-AGP ELISA Kit
201-12-1776	SunredBio	96 tests	EUR 440
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Alpha 1 Acid Glycoprotein (A1-AGP), bovine
A1AG20-N	Alpha Diagnostics	1 mg	EUR 164

Alpha 1 Acid Glycoprotein (A1-AGP), Rabbit
A1AG23-N-25	Alpha Diagnostics	25 ug	EUR 286

Alpha 1 Acid Glycoprotein (A1-AGP), Turkey
A1AG24-N-100	Alpha Diagnostics	100 ug	EUR 164

anti-α1-acid glycoprotein (AGP)(29A1)
LF-MA0167	Abfrontier	100 ul	EUR 334
Description: Mouse monoclonal to α1-acid glycoprotein (AGP)

CLIA kit for Mouse ?1-AGP (?1-Acid glycoprotein)
E-CL-M0687	Elabscience Biotech	1 plate of 96 wells	EUR 584
Description: A sandwich CLIA kit for quantitative measurement of Mouse ?1-AGP (?1-Acid glycoprotein) in samples from Serum, Plasma, Cell supernatant

CLIA kit for Rat ?1-AGP (?1-Acid Glycoprotein)
E-CL-R0695	Elabscience Biotech	1 plate of 96 wells	EUR 584
Description: A sandwich CLIA kit for quantitative measurement of Rat ?1-AGP (?1-Acid Glycoprotein) in samples from Serum, Plasma, Cell supernatant

Human Alpha-1-Acid Glycoprotein ELISA Kit (AGP 1)
RK00779	Abclonal	96 Tests	EUR 521

Sheep A1-AGP ELISA Kit
ESA0695	Abclonal	96Tests	EUR 521

Mouse A1-AGP ELISA Kit
EMA0695	Abclonal	96Tests	EUR 521

Anserini A1-AGP ELISA Kit
EAA0695	Abclonal	96Tests	EUR 521

Rabbit A1-AGP ELISA Kit
ERTA0695	Abclonal	96Tests	EUR 521

Monkey A1-AGP ELISA Kit
EMKA0695	Abclonal	96Tests	EUR 521

Porcine A1-AGP ELISA Kit
EPA0695	Abclonal	96Tests	EUR 521

Rat A1-AGP ELISA Kit
ERA0695	Abclonal	96Tests	EUR 521

Human A1-AGP ELISA Kit
EHA0695	Abclonal	96Tests	EUR 521

Canine A1-AGP ELISA Kit
ECA0695	Abclonal	96Tests	EUR 521

Chicken A1-AGP ELISA Kit
ECKA0695	Abclonal	96Tests	EUR 521

Goat A1-AGP ELISA Kit
EGTA0695	Abclonal	96Tests	EUR 521

Bovine A1-AGP ELISA Kit
EBA0695	Abclonal	96Tests	EUR 521

AGP ELISA Kit (Bovine) (OKCD06846)
OKCD06846	Aviva Systems Biology	96 Wells	EUR 1040
Description: Description of target: Functions as transport protein in the blood stream.UniRule annotation;Species reactivity: Bovine;Application: ELISA;Assay info: ;Sensitivity: < 7.0ng/mL

Human Alpha-1-Acid-Glycoprotein (Orosomucoid, AGP) Antibody
12011-05011	AssayPro	150 ug	EUR 217

Human alpha-1-Acid-Glycoprotein (Orosomucoid, AGP) Antibody
32220-05111	AssayPro	150 ug	EUR 261

Alpha 1 Acid Glycoprotein (A1-AGP), Human Plasma
A1AG15-N	Alpha Diagnostics	1 mg	EUR 164

Bovine α1-Acid glycoprotein, α1-AGP ELISA Kit
CSB-E08579b-24T	Cusabio	1 plate of 24 wells	EUR 165
Description: Quantitativecompetitive ELISA kit for measuring Bovine α1-Acid glycoprotein, α1-AGP in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Bovine α1-Acid glycoprotein, α1-AGP ELISA Kit
1-CSB-E08579b	Cusabio	EUR 703.00 EUR 4843.00 EUR 2570.00	1 plate of 96 wells 10 plates of 96 wells each 5 plates of 96 wells each
Description: Quantitativecompetitive ELISA kit for measuring Bovine α1-Acid glycoprotein, α1-AGP in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Rat α1-Acid glycoprotein(α1-AGP) ELISA Kit
CSB-E12730r-24T	Cusabio	1 plate of 24 wells	EUR 165
Description: Quantitativesandwich ELISA kit for measuring Rat α1-Acid glycoprotein (α1-AGP) in samples from serum, plasma, urine, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Rat α1-Acid glycoprotein(α1-AGP) ELISA Kit
1-CSB-E12730r	Cusabio	EUR 703.00 EUR 4843.00 EUR 2570.00	1 plate of 96 wells 10 plates of 96 wells each 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Rat α1-Acid glycoprotein(α1-AGP) in samples from serum, plasma, urine, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

ELISA kit for Rat ?1-AGP (Alpha-1-Acid Glycoprotein)
E-EL-R1081	Elabscience Biotech	1 plate of 96 wells	EUR 534
Description: A sandwich ELISA kit for quantitative measurement of Rat ?1-AGP (Alpha-1-Acid Glycoprotein) in samples from Serum, Plasma, Cell supernatant

CLIA kit for Human ?1-AGP (Alpha-1-Acid Glycoprotein)
E-CL-H0001	Elabscience Biotech	1 plate of 96 wells	EUR 584
Description: A sandwich CLIA kit for quantitative measurement of Human ?1-AGP (Alpha-1-Acid Glycoprotein) in samples from Serum, Plasma, Cell supernatant

Chicken a1-Acid Glycoprotein (a-1-AGP) ELISA Kit
QY-E80114	Qayee Biotechnology	96T	EUR 426

Alpha 1 Acid Glycoprotein (A1-AGP), Pig/Porcine/Swine
A1AG22-N-100	Alpha Diagnostics	100 ug	EUR 164

Guinea Pig A1-AGP ELISA Kit
EGA0695	Abclonal	96Tests	EUR 521

Human Alpha-1-Acid-Glycoprotein (Orosomucoid, AGP) Antibody (Biotin Conjugate)
12011-05021	AssayPro	150 ug	EUR 276

Human alpha-1-Acid-Glycoprotein (Orosomucoid, AGP) Antibody (Biotin Conjugate)
32220-05121	AssayPro	150 ug	EUR 369

Human Alpha-1-Acid-Glycoprotein (Orosomucoid, AGP) AssayLite Antibody (FITC Conjugate)
12011-05041	AssayPro	150 ug	EUR 428

Human Alpha-1-Acid-Glycoprotein (Orosomucoid, AGP) AssayLite Antibody (RPE Conjugate)
12011-05051	AssayPro	150 ug	EUR 428

Human Alpha-1-Acid-Glycoprotein (Orosomucoid, AGP) AssayLite Antibody (APC Conjugate)
12011-05061	AssayPro	150 ug	EUR 428

Human Alpha-1-Acid-Glycoprotein (Orosomucoid, AGP) AssayLite Antibody (PerCP Conjugate)
12011-05071	AssayPro	150 ug	EUR 471

Human alpha-1-Acid-Glycoprotein (Orosomucoid, AGP) AssayLite Antibody (FITC Conjugate)
32220-05141	AssayPro	150 ug	EUR 428

Human alpha-1-Acid-Glycoprotein (Orosomucoid, AGP) AssayLite Antibody (RPE Conjugate)
32220-05151	AssayPro	150 ug	EUR 428

Human alpha-1-Acid-Glycoprotein (Orosomucoid, AGP) AssayLite Antibody (APC Conjugate)
32220-05161	AssayPro	150 ug	EUR 428

Human alpha-1-Acid-Glycoprotein (Orosomucoid, AGP) AssayLite Antibody (PerCP Conjugate)
32220-05171	AssayPro	150 ug	EUR 471

Rat Alpha-1 Glycoprotein (A1-AGP) ELISA kit 96 tests, Quantitative
6490	Alpha Diagnostics	1 Kit	EUR 773

Monkey Alpha-1 Glycoprotein (A1-AGP) ELISA kit 96 tests, Quantitative
6490-10	Alpha Diagnostics	1 kit	EUR 773

Human Alpha 1-Acid Glycoprotein (A1-AGP) protein control for WB
A1AG11-C	Alpha Diagnostics	100 ul	EUR 286

Monoclonal Anti-Human Alpha 1-Acid Glycoprotein (A1-AGP) protein, ascites
A1AG12-M	Alpha Diagnostics	100 ul	EUR 482

Rat α1-Acid glycoprotein(α1-AGP)ELISA Kit
GA-E0093RT-48T	GenAsia Biotech	48T	EUR 317

Rat α1-Acid glycoprotein(α1-AGP)ELISA Kit
GA-E0093RT-96T	GenAsia Biotech	96T	EUR 496

Porcine α1-Acid glycoprotein,α1-AGP ELISA Kit
GA-E0068PC-48T	GenAsia Biotech	48T	EUR 364

Porcine α1-Acid glycoprotein,α1-AGP ELISA Kit
GA-E0068PC-96T	GenAsia Biotech	96T	EUR 590

Human α1-Acid glycoprotein(α1-AGP)ELISA Kit
GA-E1792HM-48T	GenAsia Biotech	48T	EUR 289

Human α1-Acid glycoprotein(α1-AGP)ELISA Kit
GA-E1792HM-96T	GenAsia Biotech	96T	EUR 466

Rat α1-AGP(α1-Acid Glycoprotein) ELISA Kit
ER1432	FN Test	96T	EUR 524.1
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 3.75 ng/ml

Rat α1-Acid glycoprotein(α1-AGP)ELISA Kit
QY-E11560	Qayee Biotechnology	96T	EUR 361

Mouse α1-Acid glycoprotein(α1-AGP)ELISA Kit
QY-E21637	Qayee Biotechnology	96T	EUR 361

Human α1-Acid glycoprotein(α1-AGP)ELISA Kit
QY-E04459	Qayee Biotechnology	96T	EUR 361

Mouse α1-AGP(α1-Acid glycoprotein) ELISA Kit
EM1448	FN Test	96T	EUR 524.1
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 3.75 ng/ml

Porcine α1-AGP(α1-Acid Glycoprotein) ELISA Kit
EP0214	FN Test	96T	EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Pig;Sensitivity: 0.094 ng/ml

Rat α1-Acid glycoprotein,α1-AGP ELISA Kit
CN-01787R1	ChemNorm	96T	EUR 473

Rat α1-Acid glycoprotein,α1-AGP ELISA Kit
CN-01787R2	ChemNorm	48T	EUR 323

Mouse α1-Acid glycoprotein,α1-AGP ELISA Kit
CN-02666M1	ChemNorm	96T	EUR 464

Mouse α1-Acid glycoprotein,α1-AGP ELISA Kit
CN-02666M2	ChemNorm	48T	EUR 313

Bovine α1-Acid glycoprotein,α1-AGP ELISA Kit
CN-00487B1	ChemNorm	96T	EUR 458

Bovine α1-Acid glycoprotein,α2-AGP ELISA Kit
CN-00487B2	ChemNorm	48T	EUR 307

Rabbit Anti-Human Alpha 1-Acid Glycoprotein (A1-AGP) protein IgG, unlabeled
A1AG11-A	Alpha Diagnostics	100 ul	EUR 408

Rabbit Anti-Human Alpha 1-Acid Glycoprotein (A1-AGP) protein IgG, Biotin conjugate
A1AG11-BT	Alpha Diagnostics	100 ul	EUR 408

Rabbit Anti-Human Alpha 1-Acid Glycoprotein (A1-AGP) protein IgG, HRP conjugate
A1AG11-HRP	Alpha Diagnostics	100 ul	EUR 408

Human Alpha-1-Acid-Glycoprotein (Orosomucoid, AGP) AssayLite Antibody (FITC, RPE, APC, PerCP Conjugate)
12011-05081	AssayPro	1 x 75 ug, 3 x 30 ug	EUR 585

Neuregulin/Heregulin-1? (NRG-1?/HRG-1?), human recombinant protein
P1054-1	ApexBio	1 mg	EUR 3947
Description: Neuregulin (NRG) is a signaling protein for ErbB2/ErbB4 receptor heterodimers on the cardiac muscle cells and plays an important role in heart structure and function through inducing cardiomyocyte differentiation

Angiotensin 1/2 (1-7) amide
A1054-1	ApexBio	1 mg	EUR 96
Description: Angiotensin I/II (1-7) amide (H2N-Asp-Arg-Val-Tyr-Ile-His-Pro-amide) is a peptide analog to angiotensin II that is used as a vasopressor in the treatment of certain types of shock and circulatory collapse.

Angiotensin 1/2 (1-8) amide
A1055-1	ApexBio	1 mg	EUR 96
Description: The physiologically active peptide angiotensin-2 (angiotensin 1- 8) is yielded from angiotensin-1, a substrate of ACE (angiotensin converting enzyme), by removing a dipeptide.

Endothelin-1 (1-15), amide, human
A1111-1	ApexBio	1 mg	EUR 722
Description: Endothelins are 21-amino acid vasoconstricting peptides produced primarily in the endothelium and have a key role in vascular homeostasis.

Haptoglobin, (Phenotype 1-1) Human Plasma
7536-1	Biovision		EUR 321

Flutax 1
B6986-1	ApexBio	1 mg	EUR 399

TAPI-1
B4686-1	ApexBio	1 mg	EUR 268
Description: TAPI-1 is an inhibitor of tumour necrosis factor with IC50 value of 8.09 ?M [1].TAPI-1 is an inhibitor of TACE/ADAM17 which catalyzes the cleavage of full-length APP to the soluble N-terminal fragment (sAPP?).

C14TKL-1
B5240-1	ApexBio	1 mg	EUR 399

?1-MSH
B5427-1	ApexBio	1 mg	EUR 268

MMK 1
B5446-1	ApexBio	1 mg	EUR 268

CRSP-1
B5452-.1	ApexBio	100 ug	EUR 415

ADWX 1
B5722-.1	ApexBio	100 ug	EUR 583
Description: ADWX 1 is a potent and selective inhibitor of KV1.3 channel with IC50 values of 1.89 pM and 0.65 nM for Kv1.3 and Kv1.1, respectively [1].

Purotoxin 1
B5771-.1	ApexBio	100 µg	EUR 380

Shz 1
B5772-1	ApexBio	1 mg	EUR 118

Psalmotoxin 1
B5796-.1	ApexBio	100 ug	EUR 447
Description: IC50: 0.9 nMPsalmotoxin 1 is a potent and selective acid-sensing ion channel 1a (ASIC1a) blocker.Several ASIC subunits described: ASIC1a, ASIC1b, ASIC2a, ASIC2b, and ASIC3 with different kinetics, tissue distribution, and external pH sensitivities.

UK 1
C5006-1	ApexBio	1 mg	EUR 363
Description: UK 1, an unusual bis-benzoxazole metabolite isolated from Streptomyces sp. 517-02, is an inhibitor of topoisomerase II (Topo II) and hepatitis C viral replication.

KP372-1
C5053-1	ApexBio	1 mg	EUR 132
Description: IC50: 30-60 nM for thyroid cancer cells KP372-1 is a specific Akt inhibitor. The phosphatidylinositol 3' kinase (PI3K)/phosphatase, which is a key regulator of cell proliferation and survival, is mutated or activated in various cancers.

IDE 1
B7566-1	ApexBio	1 mg	EUR 118

Rbin-1
B7823-1	ApexBio	1 mg	EUR 154
Description: Rbin-1 is an eukaryotic ribosome assembly inhibitor.All cellular proteins are synthesized by ribosomes, whose biogenesis is a complex multi-step process quickly completed in eukaryotes.

4EGI-1
B2901-1	Biovision		EUR 142

CMPD-1
B2991-1	Biovision	1mg	EUR 125