The most preferred decellularization technique in creating bioscaffolds for complex organs such as kidneys is through detergent perfusion. Detergents such as sodium dodecyl sulfate (SDS) flow to the kidneys to remove cells but using this technique alone requires long treatment times. Coupling this technique with sonication treatment decreases decellularization time but may cause damages in the microarchitecture of the kidney.
This study evaluated the effects of decellularization parameters specifically SDS concentration (0.25%, 0.625%, and 1.0%wt/vol), flowrate (15, 30, and 45 mL/min), and sonicator power (0, 60, and 120 W) on the length of time needed to produce acellular and intact bioscaffolds. Decellularization was carried out by perfusing SDS to the renal artery of the cadaveric porcine kidney.
while exposed to sonication treatment. Results showed that a significant decrease in decellularization time was observed in producing acellular scaffold when perfusion decellularization was coupled with sonication.
In addition, SDS concentration, SDS flowrate, and sonicator power had significant effects on the decellularization time while only sonicator power had a significant effect on the microarchitecture integrity of the scaffold. Lastly, H&E results showed that the produced bioscaffold showed complete cell removal with only minimal to moderate disruptions on the microarchitecture of the kidney.
Dog AGP ELISA Kit |
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E-40AGP | Immunology Consultants Laboratory | 1 x 96 well plate | EUR 590.4 |
Cow AGP ELISA kit |
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AGP-11 | Life Diagnostics | 1x 96 wells plate | EUR 475 |
Rat AGP ELISA kit |
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AGP-2 | Life Diagnostics | 1x 96 wells plate | EUR 425 |
Dog AGP ELISA kit |
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AGP-4 | Life Diagnostics | 1x 96 wells plate | EUR 475 |
Cat AGP ELISA kit |
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AGP-8 | Life Diagnostics | 1x 96 wells plate | EUR 475 |
Pig AGP ELISA kit |
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AGP-9 | Life Diagnostics | 1x 96 wells plate | EUR 475 |
Mouse AGP ELISA kit |
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AGP-1 | Life Diagnostics | 1x 96 wells plate | EUR 475 |
Monkey AGP ELISA kit |
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AGP-3 | Life Diagnostics | 1x 96 wells plate | EUR 475 |
Turkey AGP ELISA kit |
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AGP-6 | Life Diagnostics | 1x 96 wells plate | EUR 450 |
Chicken AGP ELISA kit |
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AGP-5 | Life Diagnostics | 1x 96 wells plate | EUR 475 |
Human ?1-Acid glycoprotein,?1-AGP ELISA Kit |
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201-12-1776 | SunredBio | 96 tests | EUR 528 |
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. |
anti-α1-acid glycoprotein (AGP)(29A1) |
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LF-MA0167 | Abfrontier | 100 ul | EUR 400.8 |
Description: Mouse monoclonal to α1-acid glycoprotein (AGP) |
Canine Alpha 1-Acid Glycoprotein (AGP) ELISA |
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GWB-374Z17 | GenWay Biotech | 96 well | Ask for price |
Chicken Alpha 1-Acid Glycoprotein (AGP) ELISA |
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GWB-374Z11 | GenWay Biotech | 96 well | Ask for price |
1 AGP acyltransferase 4 (AGPAT4) rabbit polyclonal antibody |
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AP17875PU-N | Origene Technologies GmbH | 400 µl | Ask for price |
Cow AGP SPARCL™ Assay |
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AGP-SP-11 | Life Diagnostics | 1 kit | EUR 400 |
Rat AGP SPARCL™ Assay |
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AGP-SP-2 | Life Diagnostics | 1 kit | EUR 500 |
Cat AGP SPARCL™ Assay |
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AGP-SP-8 | Life Diagnostics | 1 kit | EUR 500 |
Chicken AGP SPARCL™ Assay |
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AGP-SP-5 | Life Diagnostics | 1 kit | EUR 500 |
CLIA kit for Rat ?1-AGP (?1-Acid Glycoprotein) |
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E-CL-R0695 | Elabscience Biotech | 1 plate of 96 wells | EUR 700.8 |
Description: A sandwich CLIA kit for quantitative measurement of Rat ?1-AGP (?1-Acid Glycoprotein) in samples from Serum, Plasma, Cell supernatant |
CLIA kit for Mouse ?1-AGP (?1-Acid glycoprotein) |
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E-CL-M0687 | Elabscience Biotech | 1 plate of 96 wells | EUR 700.8 |
Description: A sandwich CLIA kit for quantitative measurement of Mouse ?1-AGP (?1-Acid glycoprotein) in samples from Serum, Plasma, Cell supernatant |
AGP ELISA Kit (Bovine) (OKCD06846) |
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OKCD06846 | Aviva Systems Biology | 96 Wells | EUR 1248 |
Description: Description of target: Functions as transport protein in the blood stream.UniRule annotation;Species reactivity: Bovine;Application: ELISA;Assay info: ;Sensitivity: < 7.0ng/mL |
Human Alpha-1-Acid Glycoprotein ELISA Kit (AGP 1) |
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RK00779 | Abclonal | 96 Tests | EUR 625.2 |
Bovine Orosomucoid 1,ORM1/α1-AGP ELISA Kit |
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YLA0129BO-48T | Shanghai YL Biotech | 48T | Ask for price |
Bovine Orosomucoid 1,ORM1/α1-AGP ELISA Kit |
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YLA0129BO-96T | Shanghai YL Biotech | 96T | Ask for price |
Rabbit Orosomucoid 1,ORM1/α1-AGP ELISA Kit |
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YLA0199RB-48T | Shanghai YL Biotech | 48T | Ask for price |
Rabbit Orosomucoid 1,ORM1/α1-AGP ELISA Kit |
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YLA0199RB-96T | Shanghai YL Biotech | 96T | Ask for price |
Rat Alpha-1-Glycoprotein (α1-AGP) ELISA Kit |
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SL0760Ra | Sunlong | - | EUR 498 |
Rabbit Orosomucoid 1, ORM1/α1-AGP GENLISA ELISA |
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KLX0128 | Krishgen | 1 x 96 wells | EUR 341 |
Bovine Orosomucoid 1, ORM1/α1-AGP GENLISA ELISA |
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KLB0201 | Krishgen | 1 x 96 wells | EUR 341 |
OPCD00959-10UG - AGP Recombinant Protein |
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OPCD00959-10UG | Aviva Systems Biology | 10ug | EUR 149 |
OPCD00959-50UG - AGP Recombinant Protein |
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OPCD00959-50UG | Aviva Systems Biology | 50ug | EUR 379 |
OPCD00959-200UG - AGP Recombinant Protein |
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OPCD00959-200UG | Aviva Systems Biology | 200ug | EUR 749 |
Rat α1-Acid glycoprotein(α1-AGP) ELISA Kit |
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CSB-E12730r-24T | Cusabio | 1 plate of 24 wells | EUR 198 |
Description: Quantitativesandwich ELISA kit for measuring Rat α1-Acid glycoprotein (α1-AGP) in samples from serum, plasma, urine, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price. |
Rat α1-Acid glycoprotein(α1-AGP) ELISA Kit |
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1-CSB-E12730r | Cusabio |
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Description: Quantitativesandwich ELISA kit for measuring Rat α1-Acid glycoprotein(α1-AGP) in samples from serum, plasma, urine, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits. |
Cat AGP ELISA Kit | E-20AGP |
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E-20AGP | Immunology Consultants Laboratory | Kit 1 x 96 well plate | EUR 760 |
Description: Cat AGP ELISA Kit ; E-20AGP ; Immunology Consultants Laboratory Species Reactivity: Cat Format: Sandwich ELISA Product Type: ELISA Kit Range: 1.57 ng/ml - 100 ng/ml |
Rat AGP ELISA Kit | E-25AGP |
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E-25AGP | Immunology Consultants Laboratory | 1 x 96 well plate | EUR 680 |
Description: Rat AGP ELISA Kit ; E-25AGP ; Immunology Consultants Laboratory Species Reactivity: Rat Format: Sandwich ELISA Product Type: ELISA Kit Range: 12.5 ng/ml - 400 ng/ml |
Bovine α1-Acid glycoprotein, α1-AGP ELISA Kit |
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CSB-E08579b-24T | Cusabio | 1 plate of 24 wells | EUR 198 |
Description: Quantitativecompetitive ELISA kit for measuring Bovine α1-Acid glycoprotein, α1-AGP in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price. |
Bovine α1-Acid glycoprotein, α1-AGP ELISA Kit |
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1-CSB-E08579b | Cusabio |
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Description: Quantitativecompetitive ELISA kit for measuring Bovine α1-Acid glycoprotein, α1-AGP in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits. |
Nori® Monkey AGP ELISA Kit |
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GR169206 | Genorise Scientific | 96-well | EUR 461 |
Nori® Ferret AGP ELISA Kit |
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GR201130 | Genorise Scientific | 96-well | EUR 461 |
Nori® Salmon AGP ELISA Kit |
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GR202130 | Genorise Scientific | 96-well | EUR 461 |
Human alpha-1-Acid-Glycoprotein (Orosomucoid, AGP) Antibody |
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12011-05011 | AssayPro | 150 ug | EUR 175 |
Human alpha-1-Acid-Glycoprotein (Orosomucoid, AGP) Antibody |
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32220-05111 | AssayPro | 150 ug | EUR 313.2 |
Human AGP ELISA Kit | E-80AGP |
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E-80AGP | Immunology Consultants Laboratory | 1 x 96 well plate | EUR 570 |
Description: Human AGP ELISA Kit ; E-80AGP ; Immunology Consultants Laboratory Species Reactivity: Human Format: Sandwich ELISA Product Type: ELISA Kit Range: 5 ng/ml - 320 ng/ml |
Nori® Hamster AGP ELISA Kit |
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GR172256 | Genorise Scientific | 96-well | EUR 461 |
Monkey alpha-1-Acid-Glycoprotein (Orosomucoid, AGP) Antibody |
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36816-05111 | AssayPro | 150 ug | EUR 215 |
Bovine AGP ELISA Kit | E-10AGP |
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E-10AGP | Immunology Consultants Laboratory | 1 x 96 well plate | EUR 712 |
Description: Bovine AGP ELISA Kit ; E-10AGP ; Immunology Consultants Laboratory Species Reactivity: Bovine Format: Sandwich ELISA Product Type: ELISA Kit Range: 3.125 ng/ml - 100 ng/ml |
ELISA kit for Rat ?1-AGP (Alpha-1-Acid Glycoprotein) |
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E-EL-R1081 | Elabscience Biotech | 1 plate of 96 wells | EUR 640.8 |
Description: A sandwich ELISA kit for quantitative measurement of Rat ?1-AGP (Alpha-1-Acid Glycoprotein) in samples from Serum, Plasma, Cell supernatant |
Chicken AGP ELISA Kit | E-30AGP |
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E-30AGP | Immunology Consultants Laboratory | 1 x 96 well plate | EUR 680 |
Description: Chicken AGP ELISA Kit ; E-30AGP ; Immunology Consultants Laboratory Species Reactivity: Chicken Format: Sandwich ELISA Product Type: ELISA Kit Range: 6.25 ng/ml - 200 ng/ml |
Nori® Zebrafish AGP ELISA Kit |
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GR206130 | Genorise Scientific | 96-well | EUR 461 |
CLIA kit for Human ?1-AGP (Alpha-1-Acid Glycoprotein) |
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E-CL-H0001 | Elabscience Biotech | 1 plate of 96 wells | EUR 700.8 |
Description: A sandwich CLIA kit for quantitative measurement of Human ?1-AGP (Alpha-1-Acid Glycoprotein) in samples from Serum, Plasma, Cell supernatant |
Human Alpha-1-Acid Glycoprotein(α 1-AGP) ELISA Kit |
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NSL1063Hu | Sunlong | 96 wells | EUR 468 |
Bovine Alpha-1-Acid Glycoprotein(α 1-AGP) ELISA Kit |
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NSL1864Bo | Sunlong | 96 T | EUR 528 |
Canine Alpha-1-Acid Glycoprotein(α 1-AGP) ELISA Kit |
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NSL2023Ca | Sunlong | 2118 wells | EUR 528 |
Porcine Alpha-1-Acid Glycoprotein(α 1-AGP) ELISA Kit |
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NSL1417Po | Sunlong | 96T | EUR 528 |
OKCD06846-96W - AGP ELISA Kit (Bovine) |
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OKCD06846-96W | Aviva Systems Biology | 96Wells | EUR 850 |
Rat Alpha-1-Acid Glycoprotein (α1-AGP)ELISA Kit |
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NSL1641r | Sunlong | 96 Tests | EUR 498 |
Goat Alpha-1-Acid Glycoprotein (α1-AGP)ELISA Kit |
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NSL1164Gt | Sunlong | 96T | EUR 528 |
Mouse Alpha-1-Acid Glycoprotein (α1-AGP) ELISA Kit |
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NSL1616m | Sunlong | 96 Test | EUR 528 |
Canine Alpha-1-Acid Glycoprotein (α1-AGP)ELISA Kit |
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NSL0049Ca | Sunlong | 144 wells | EUR 528 |
Bovine Alpha-1-Acid glycoprotein (α1-AGP) ELISA Kit |
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RK00434 | Abclonal | 96 Tests | EUR 280 |
Chicken Alpha-1-Acid Glycoprotein (α1-AGP)ELISA Kit |
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NSL1202Ch | Sunlong | 1297 T | EUR 528 |
Guinea pig Alpha-1-Acid Glycoprotein(α 1-AGP) ELISA Kit |
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NSL1468Gp | Sunlong | 96T | EUR 528 |
Nori® Mouse Alpha 1 Acid Glycoprotein (AGP) ELISA Kit |
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GR117259 | Genorise Scientific | 96-well | EUR 461 |
Nori® Rabbit α-1-acid glycoprotein (AGP) ELISA Kit |
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GR144192 | Genorise Scientific | 96-well | EUR 461 |
Nori® Rat alpha-1-acid glycoprotein (AGP) ELISA Kit |
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GR118215 | Genorise Scientific | 96-well | EUR 461 |
Nori® Human alpha-1-acid glycoprotein (AGP) ELISA Kit |
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GR111268 | Genorise Scientific | 96-well | EUR 461 |
Nori® Sheep alpha-1-acid glycoprotein (AGP) ELISA Kit |
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GR116243 | Genorise Scientific | 96-well | EUR 461 |
Nori® Equine alpha-1-acid Glycoprotein (AGP) ELISA Kit |
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GR106790 | Genorise Scientific | 96-well | EUR 461 |
Nori® Canine alpha-1-acid glycoprotein (AGP) ELISA Kit |
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GR115288 | Genorise Scientific | 96-well | EUR 461 |
Nori® Feline alpha-1-acid glycoprotein (AGP) ELISA Kit |
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GR188218 | Genorise Scientific | 96-well | EUR 461 |
Nori® Bovine alpha-1-acid Glycoprotein (AGP) ELISA Kit |
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GR112256 | Genorise Scientific | 96-well | EUR 461 |
Nori® Chicken alpha-1-acid glycoprotein (AGP) ELISA Kit |
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GR114102 | Genorise Scientific | 96-well | EUR 461 |
Nori® Porcine alpha-1-acid glycoprotein (AGP) ELISA Kit |
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GR113282 | Genorise Scientific | 96-well | EUR 461 |
Human Alpha-1-Acid Glycoprotein (Orosomucoid, AGP) AssayMax ELISA Kit |
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EG5101-1 | AssayPro | 96 Well Plate | EUR 475.2 |
Human alpha-1-Acid-Glycoprotein (Orosomucoid, AGP) Antibody (Biotin Conjugate) |
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12011-05021 | AssayPro | 150 ug | EUR 229 |
Human alpha-1-Acid-Glycoprotein (Orosomucoid, AGP) Antibody (Biotin Conjugate) |
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32220-05121 | AssayPro | 150 ug | EUR 442.8 |
Monkey alpha-1-Acid-Glycoprotein (Orosomucoid, AGP) Antibody (Biotin Conjugate) |
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36816-05121 | AssayPro | 150 ug | EUR 315 |
Nori® Guinea Pig alpha-1-acid Glycoprotein (AGP) ELISA Kit |
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GR171187 | Genorise Scientific | 96-well | EUR 461 |
Rat α1-Acid glycoprotein(α1-AGP) Elisa Kit |
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EK720917 | AFG Bioscience LLC | 96 Wells | EUR 0.62 |
Rat α1-Acid glycoprotein(α1-AGP) ELISA Kit |
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EKC42303-48T | Biomatik Corporation | 48T | EUR 474.04 |
Rat α1-Acid glycoprotein(α1-AGP) ELISA Kit |
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EKC42303-5x96T | Biomatik Corporation | 5x96T | EUR 3216.7 |
Rat α1-Acid glycoprotein(α1-AGP) ELISA Kit |
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EKC42303-96T | Biomatik Corporation | 96T | EUR 677.2 |
Rat α1-Acid glycoprotein,α1-AGP ELISA Kit |
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CN-01787R1 | ChemNorm | 96T | EUR 567.6 |
Rat α1-Acid glycoprotein,α1-AGP ELISA Kit |
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CN-01787R2 | ChemNorm | 48T | EUR 387.6 |
Rat α1-AGP(α1-Acid Glycoprotein) ELISA Kit |
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ER1432 | FN Test | 96T | EUR 628.92 |
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 3.75 ng/ml |
Rat α1-Acid glycoprotein(α1-AGP)ELISA Kit |
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GA-E0093RT-48T | GenAsia Biotech | 48T | EUR 380.4 |
Rat α1-Acid glycoprotein(α1-AGP)ELISA Kit |
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GA-E0093RT-96T | GenAsia Biotech | 96T | EUR 595.2 |
Rat α1-Acid glycoprotein(α1-AGP)ELISA Kit |
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QY-E11560 | Qayee Biotechnology | 96T | EUR 433.2 |
Rat α1-Acid glycoprotein,α1-AGP ELISA Kit |
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YLA0580RA-48T | Shanghai YL Biotech | 48T | Ask for price |
Rat α1-Acid glycoprotein,α1-AGP ELISA Kit |
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YLA0580RA-96T | Shanghai YL Biotech | 96T | Ask for price |
GOAT α1-Acid glycoprotein(α1-AGP)ELISA Kit |
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YLA0043GO-48T | Shanghai YL Biotech | 48T | Ask for price |
GOAT α1-Acid glycoprotein(α1-AGP)ELISA Kit |
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YLA0043GO-96T | Shanghai YL Biotech | 96T | Ask for price |
Mouse α1-Acid glycoprotein,α1-AGP Elisa Kit |
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EK730369 | AFG Bioscience LLC | 96 Wells | EUR 0.68 |
Human α1-Acid glycoprotein(α1-AGP) Elisa Kit |
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EK711545 | AFG Bioscience LLC | 96 Wells | EUR 0.18 |
Mouse α1-Acid glycoprotein,α1-AGP ELISA Kit |
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CN-02666M1 | ChemNorm | 96T | EUR 556.8 |
Mouse α1-Acid glycoprotein,α1-AGP ELISA Kit |
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CN-02666M2 | ChemNorm | 48T | EUR 375.6 |
Human α1-Acid glycoprotein(α1-AGP)ELISA Kit |
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GA-E1792HM-48T | GenAsia Biotech | 48T | EUR 346.8 |
Human α1-Acid glycoprotein(α1-AGP)ELISA Kit |
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GA-E1792HM-96T | GenAsia Biotech | 96T | EUR 559.2 |